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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB IHC IF
- 靶点:
Uniprot:P62753
- 抗体英文名:
S6 Ribosomal Protein Antibody
- 规格:
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 32kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
图片:

Western blot analysis of extracts from HepG2 and 293 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.
,

Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using S6 Ribosomal Protein(Ab-235) Antibody #21225(left) or the same antibody preincubated with blocking peptide(right).
,

Immunofluorescence staining of methanol-fixed MCF7 cells using S6 Ribosomal Protein(Ab-235) Antibody #21225.
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文献和实验构成核糖体的蛋白质。大肠杆菌核糖体蛋白的初级结构均被确定。大肠杆菌核糖体的 30S亚基含 S1 — S21 共 21种蛋白质, 50S亚基含 L1— L34共 34种蛋白质。这些蛋白质已被全部分离纯化。分子量约 1万到 3万。除 S6 、 L7 、 L12 之外全是碱性蛋白质。这些蛋白质是免疫学上独立的蛋白质,只有 L7 、 L1 2 显示出相互交叉反应。已知 L7 与 L1 2 是同一蛋白质, L7 的 N末端被乙酰化。已经确定了几种蛋白的一级结构。机能已经
PCR and RFLP Analyses Based on the Ribosomal Protein Operon
. Classification of phytoplasma strains can, however, become more refined and specific if moderately conserved genes, such as the ribosomal protein (rp ) genes, are used as genetic markers. The use of additional genetic markers enhances the resolving power
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
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