- 询价
- Signalway Antibody
- 21173
- 2025年09月03日
- WB IHC
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC
- 靶点：
Uniprot：P43403
- 抗体英文名：
Zap-70 Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
图片:
Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody #21173.
,
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70(Ab-319) Antibody #21173(left) or the same antibody preincubated with blocking peptide(right).
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文献和实验70% Ethanol Fixation for Recovery of DNA, RNA, and Protein This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following
Clone Excision Methods for the Lambda ZAP-Based Vectors
The Lambda ZAP� vectors have been designed to allow in vivo excision and recircularization of the cloned insert and phagemid sequences contained within the λ vector (1 ). Once a Lambda ZAP library is constructed and amplified, putative
cDNA Library Construction for the Lambda ZAP-Based Vectors
Because the vast majority of plant viruses have a positive-sense RNA genome, which acts as the viral mRNA, the RNA must first be converted into cDNA before cloning, amplification, and subsequent manipulation. Successful cDNA synthesis
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