- ￥1000
- abinscience
- HA135013
- 2025年09月02日
- IHC, WB
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 保存条件：
-20℃
- 抗原来源：
Mouse
- 目录编号：
HA135013
- 供应商：
广州市左克生物
- 宿主：
Mouse
- 应用范围：
IHC, WB
- 靶点：
Potassium voltage-gated channel subfamily G member 4, Voltage-gated potassium channel subunit Kv6.4, KCNG3, KCNG4
- 抗体英文名：
Anti-Kv6.4 K+ channel Antibody (N458/10)
- 抗体名：
Anti-Kv6.4 K+ channel Antibody (N458/10)
- 规格：
50ug
产品简介:
品牌：abinscience
货号：HA135013
产品名称：Anti-Kv6.4 K+ channel Antibody (N458/10)
https://www.abinscience.com/product/34/17844.html
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
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