Sigma货号P2297苦基磺酸溶液(Picrylsulfo
nic acid solution)(别名:2,4,6-三硝(xiāo)基苯磺酸溶液，TNBS)上海睿安生物19121878899- ￥956.50
- Sigma-Aldrich
- P2297
- made in USA
- 2026年03月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100⁺瓶
- 英文名：
Picrylsulfonic acid solution(别名:TNBS)
- CAS号：
2508-19_2
- 保质期：
1年
- 供应商：
上海睿安生物13611631389
- 保存条件：
2-8°C
- 规格：
10ml/瓶
Sigma货号P2297苦基磺酸溶液(Picrylsulfonic acid solution)(别名:2,4,6-三硝(xiāo)基苯磺酸溶液，TNBS)上海睿安生物19121878899
苦基磺酸溶液
5%(w/v) in H2O,BioReagent,suitable for determination of primary amines
别名:2,4,6-三硝(xiāo)基苯磺酸溶液，TNBS
说明
一般描述
应用
- to assess the efficiency of carboxymethyl lysine (CML) modifications of exposed primary amines
- to incubate zona intact bovine embryos for the differential staining of blastocysts
- to induce irritable bowel syndrome (IBS) in mice and to study its effects on Clostridium butyricum
常见问题
-
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
-
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
-
Is Product P2297, Picrylsulfonic acid solution, supposed to be yellow?
Yes. This product is a clear to slightly hazy yellow solution at room temperature. The solution has a concentration of 5 w/v % in H2O (5g per 100 mL).
-
In general, what is Product P2297, Picrylsulfonic acid solution, used for?
Picrylsulfonic acid has been used 1) As a hydrophilic modifying reagent[1]; 2) To make derivatives with amino compounds, which can be regenerated with hydrazine [2].
-
How is Product P2297, Picrylsulfonic acid solution, tested? Do you have any references for its use besides Sigma's method?
Sigma's suitability assay is based on a published method.1 We use a 500-fold dilution as a working solution, with glycine as a standard. Hydrochloric acid is used to stop the reaction, and to reduce yellow background due to picric acid. Sodium phosphate is used as a buffer in place of sodium bicarbonate to avoid formation of CO2 when HCl is added. Absorbance is read at 420 nm.Other procedures have been published. For example:---Twenty-five mL of 0.03 M TNBS is added to 1 mL of sample (in borate buffer), agitated to ensure complete mixing, and allowed to stand for 30 minutes at room temperature (25±1°C). No quenching step is used. In the blank, buffer replaces sample. Absorbance is read at 420 nm.2---Protocols using a more concentrated solution of picric acid (1.1 M) have been published.3Note: TNBS forms an orange complex with amines, monitored at 420 nm, but the EmM of the complexes will vary with the reacting group: for alpha-amino groups, 22.0; for epsilon-amino groups, somewhat lower; and for thiol groups, 2.25.3References:1. Satake, K. et al., J. Biochem., 47, 654 (1960).2. Snyder, S.L. and Sobocinski P.Z., Anal. Biochem., 64, 284-288 (1975).3. Fields, R., Methods in Enzymology, 25, 464-468 (1972).
-
What are the suggested storage conditions for Product P2297, Picrylsulfonic acid solution?
Store Product No. P2297 at 2-8°C in the dark.
-
Is Product P2297, Picrylsulfonic acid solution, considered to be stable?
It is explosive when it is dry. The solution should be stored at 2-8°C in the dark. Under these conditions less than 5% decomposition will occur over five months. At 37°C, 50% decomposition occurred in 60 days. Picrylsulfonic acid decomposes to picric acid, crystals of which may form in the solution, but this impurity causes no interference in usage. The solution may also contain a trace amount of 2,4-dinitrophenol (typically <0.5%). Storage at -20°C may enhance shelf-life of stock solutions.
-
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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文献和实验Sigma货号P2297苦基磺酸溶液(Picrylsulfonic acid solution)(别名:2,4,6-三硝(xiāo)基苯磺酸溶液，TNBS)上海睿安生物19121878899
苦基磺酸溶液
5%(w/v) in H2O,BioReagent,suitable for determination of primary amines
别名:2,4,6-三硝(xiāo)基苯磺酸溶液，TNBS
说明
一般描述
应用
- to assess the efficiency of carboxymethyl lysine (CML) modifications of exposed primary amines
- to incubate zona intact bovine embryos for the differential staining of blastocysts
- to induce irritable bowel syndrome (IBS) in mice and to study its effects on Clostridium butyricum
常见问题
-
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
-
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
-
Is Product P2297, Picrylsulfonic acid solution, supposed to be yellow?
Yes. This product is a clear to slightly hazy yellow solution at room temperature. The solution has a concentration of 5 w/v % in H2O (5g per 100 mL).
-
In general, what is Product P2297, Picrylsulfonic acid solution, used for?
Picrylsulfonic acid has been used 1) As a hydrophilic modifying reagent[1]; 2) To make derivatives with amino compounds, which can be regenerated with hydrazine [2].
-
How is Product P2297, Picrylsulfonic acid solution, tested? Do you have any references for its use besides Sigma's method?
Sigma's suitability assay is based on a published method.1 We use a 500-fold dilution as a working solution, with glycine as a standard. Hydrochloric acid is used to stop the reaction, and to reduce yellow background due to picric acid. Sodium phosphate is used as a buffer in place of sodium bicarbonate to avoid formation of CO2 when HCl is added. Absorbance is read at 420 nm.Other procedures have been published. For example:---Twenty-five mL of 0.03 M TNBS is added to 1 mL of sample (in borate buffer), agitated to ensure complete mixing, and allowed to stand for 30 minutes at room temperature (25±1°C). No quenching step is used. In the blank, buffer replaces sample. Absorbance is read at 420 nm.2---Protocols using a more concentrated solution of picric acid (1.1 M) have been published.3Note: TNBS forms an orange complex with amines, monitored at 420 nm, but the EmM of the complexes will vary with the reacting group: for alpha-amino groups, 22.0; for epsilon-amino groups, somewhat lower; and for thiol groups, 2.25.3References:1. Satake, K. et al., J. Biochem., 47, 654 (1960).2. Snyder, S.L. and Sobocinski P.Z., Anal. Biochem., 64, 284-288 (1975).3. Fields, R., Methods in Enzymology, 25, 464-468 (1972).
-
What are the suggested storage conditions for Product P2297, Picrylsulfonic acid solution?
Store Product No. P2297 at 2-8°C in the dark.
-
Is Product P2297, Picrylsulfonic acid solution, considered to be stable?
It is explosive when it is dry. The solution should be stored at 2-8°C in the dark. Under these conditions less than 5% decomposition will occur over five months. At 37°C, 50% decomposition occurred in 60 days. Picrylsulfonic acid decomposes to picric acid, crystals of which may form in the solution, but this impurity causes no interference in usage. The solution may also contain a trace amount of 2,4-dinitrophenol (typically <0.5%). Storage at -20°C may enhance shelf-life of stock solutions.
-
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
人酸性成纤维生长因子(FGF-acid)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人酸性成纤维生长因子 ( FGF - acid )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 FGF-acid 单抗包被于酶标板上，标准品和样品中的 FGF-acid与单抗结合，加入
或酶作用，使得基质释放出配体或配体的裂解物进行分析。5.2,4,6－三硝基苯磺酸钠（TNBS）分析：利用TNBS与未结合配体和结合某些配体的基质作用呈现不同的颜色，可以计算出配体结合量6.元素分析：如果配体中含有某种特别的元素，通过元素分析就可以确定配体结合量。7.放射性分析法：偶联中加入一定量带有同位素的配体，通过放射性分析确定配体结合量，这是一种非常灵敏的方法。影响配体结合量的因素很多，包括基质和配体的性质、基质的活化方法及条件、基质和配体偶联反应的条件等等。例如通常溴化氰活化的基质的活性
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