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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10ml
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
communication) (1ul 2.65% Polystyrene 0.1 um in water) are added to the center of the pad followed by 3-5 worms oriented so they are lying on their left sides. A glass coverslip is applied and then Vaseline is used to seal the coverslip and prevent evaporation
Sodium Pyruvate6mL 100x P/S/G500mL DMEMIBMX Solution (make fresh)a)Dissolve IBMX in a solution made of 0.5N KOH to a final concentration of 0.0115g/mL.b)Filter sterilize through a 0.22 mm syringe filter.Insulin Stock Solution 167 uM (1mg/mL) in 0.02M HCl
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
containing PCR Lysis Buffer (50 ul + Proteinase K), lysing the cells either individually or in appropriately mixed pools. 8. Using the multi-channel pipetter, add 100 ul of filter-sterilized (0.22 um) Light Paraffin
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