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- 百欧泰
- ABT-8-3000-NR3+
- 2025年08月22日
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- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10ml
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
communication) (1ul 2.65% Polystyrene 0.1 um in water) are added to the center of the pad followed by 3-5 worms oriented so they are lying on their left sides. A glass coverslip is applied and then Vaseline is used to seal the coverslip and prevent evaporation
solution)to microcentrifuge tube. 2.Add 2.5x 10,000 particles of 1 uM Fluosphere beads. 3.Mix overnight at 4 ℃ with 200 µl 5mg/ml 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. 4.Wash with PBS,by centrifuging,repeat wash 3 times. 5.Resuspend the bead
: a) to the 40 ul of digested cDNA add: 1 ul Taq I adaptor (45uM) 1 ul Nco I adaptor (5uM) 2.5 ul 10 mM ATP 0.5 ul NEB T4 DNA ligase buffer 0.2 ul T4 DNA ligase 45 ul total b) 37C for 3hrs or ON @ 30C
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