- ￥4300
- Solarbio|ActivAb
- 北京
- K011600M
- 2025年08月22日
- WB
- Mouse
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of His tag
- 亚型：
IgG
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Species independent
- 抗原来源：
A synthetic peptide of His tag
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
WB 1:5000-50000.
- 靶点：
His Tag
- 抗体英文名：
Anti-His Tag(AP) Monoclonal Antibody
- 抗体名：
His Tag标签抗体
- 规格：
100ul
| 基本信息 | |
| 英文名称 | Anti-His Tag(AP) Monoclonal Antibody |
| 中文名称 | His Tag标签抗体 |
| 宿主 | Mouse |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Species independent |
| 应用 | WB |
| 稀释比例 | WB 1:5000-50000. |
| 密度 | |
| 靶标 | His Tag |
| 别名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
| 背景说明 | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
| 免疫原 | A synthetic peptide of His tag |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验Monoclonal Antibody Production Protocol
. This could take from a few seconds to 20 min. If needed, stop the reaction by adding 50 ul of 4M NaOH. Read absorption in Elisa reader at correct wavelength (for HRP system 416 nm, for AP - 405 nm). Footnote: This is the system we have been using
Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation
of therapeutic monoclonal antibodies in the fields of transplantation and autoimmunity, and discuss the salient features of antibodies presently used in the clinic-notably anti-T-cell and anticytokine antibodies. The discussion will also focus on the unique
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