- ￥1700 - 2850
- Solarbio|ActivAb
- 北京
- K011428P
- 2025年08月22日
- WB;FCM;IF;IHC
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of human phospho-NFKB p65 (Ser468)
- 亚型：
IgG
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Polyclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Human;Mouse;Rat
- 抗原来源：
A synthetic peptide of human phospho-NFKB p65 (Ser468)
- 目录编号：
5970
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;FCM;IF;IHC
- 浓度：
WB 1:100-200. IHC 1:100-500. IF 1:100-200.FCM 1μg /test.
- 靶点：
Phospho-NFKB p65 (Ser468)
- 抗体英文名：
Anti-Phospho-NFKB p65 (Ser468) Polyclonal Antibody
- 抗体名：
磷酸化细胞核因子NF-κB p65抗体
- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥2850.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1700.0 |
| 基本信息 | |
| 英文名称 | Anti-Phospho-NFKB p65 (Ser468) Polyclonal Antibody |
| 中文名称 | 磷酸化细胞核因子NF-κB p65抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Human;Mouse;Rat |
| 预测交叉反应 | Pig;Cow;Dog;Horse |
| 应用 | WB;FCM;IF;IHC |
| 稀释比例 | WB 1:100-200. IHC 1:100-500. IF 1:100-200.FCM 1μg /test. |
| 密度 | |
| 靶标 | Phospho-NFKB p65 (Ser468) |
| 别名 | NF-kB p65 (phospho S468); p-NF-kB p65 (phospho S468); RELA(phospho S468); NF kB P65; NF-kB p65; NFKBp65; NF-κBp65; p65 NF kappaB; p65 NFkB; NFKBp65; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells. |
| 背景说明 | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| Swiss Prot | Q04206 |
| Gene ID | 5970 |
| 亚细胞定位 | Nucleus Cytoplasm |
| 免疫原 | A synthetic peptide of human phospho-NFKB p65 (Ser468) |
| 理论分子量 | 61kDa |
| 实际分子量 | 61kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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