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- 详细信息
- 文献和实验
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- 供应商:
广州市左克生物
- 规格:
96 T
产品简介:
品牌:达优
货号:2111002
产品名称:Human IL-10 Precoated ELISPOT Kit
产品规格:96 T
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1)所有产品仅可用于科研目的的生物学实验(forresearchuseonly),严禁用于人体或动物的医疗目的
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文献和实验Human ELISPOT Protocol Human ELISPOT Protocol(TNFa,IL13, IL12,IL10, IL6,IL5,IL4,IL2,IL1b,IFNg ,FasL) the protocol is in the file below:
, cryopreserving, and thawing human PBMCs; and finally, detecting vaccinia-specific IL-10 and IFNγ secreting lymphocytes, as well as CD8+ IFNγ T cells following in vitro stimulation of PBMCs with vaccinia virus. The methods presented below, although optimized
in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
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