- ￥1200 - 1500
- 富衡生物
- 自产
- FH0328
- 2026年03月27日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
RAW264.7; RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
- 库存：
复苏：1-2周。冻存：现货。（建议:复苏为主，极个别特殊情况选冻存）
- 供应商：
上海富衡生物科技有限公司
- 肿瘤类型：
小鼠巨噬细胞白血病
- 细胞类型：
真核细胞
- 品系：
BALB/c
- 组织来源：
腹腔腹水瘤
- 相关疾病：
正常巨噬细胞（无特定疾病关联，常用于免疫研究）
- 物种来源：
小鼠
- 免疫类型：
免疫细胞系（巨噬细胞，天然免疫相关）
- 细胞形态：
不规则圆形
- 是否是肿瘤细胞：
是
- 器官来源：
骨髓
- 运输方式：
常温/干冰，快递运输
- 年限：
数年
- 生长状态：
贴壁生长
- 规格：
1 × 10⁶ cells/T25 flask/冻存管
| 规格： | 1 × 10⁶ cells/T25 flask | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 冻存管 | 产品价格： | ￥1500.0 |
RAW264.7是小鼠单核巨噬细胞白血病细胞系，来源于BALB/c小鼠的腹水瘤细胞。该细胞系常用于免疫学、炎症反应、吞噬机制及癌症相关研究，具有贴壁生长特性，可分化成巨噬细胞样表型。经种属鉴定确认其小鼠源性，确保实验准确性。
| 产品名称 | 小鼠单核巨噬细胞白血病细胞；RAW264.7(STR) |
|---|---|
| 品牌 | FuHeng |
| 英文名 | RAW264.7 |
| 货号 | FH0328 |
| 规格 | 1 × 10⁶ cells/T25 flask |
| 培养 | DMEM + 10% FBS + 1% P/S |
| 别称 | RAW264.7; RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7 |
| 形态特征 | 不规则圆形 |
| 生长特性 | 贴壁生长 |
| 培养基 | RAW264.7完全培养基 |
| 培养条件 | 气相：95% 空气，5% CO₂；温度：37 ℃ |
| 传代方法 | 1:2传代 |
| 组织来源 | 腹腔腹水瘤 |
| 产品分类 | 小鼠细胞系 |
| 特别声明 | 富衡细胞库承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，细胞库资料不保证其准确性。 |
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- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
| 论文标题 | DOI | 发表时间 | 期刊 | 影响因子 | 作者列表 |
| Rapid chemical characterization of finger millet using UHPLC-Q-Exactive orbitrap MS coupled with based via Feature-Based molecular networking and PRM | 10.1038/s41598-026-35395-8 | 2026-01-22 | Scientific Reports | 3.9 | Zou Yufeng, Xiao Shunli, Wang Jiayi, Chen Qiru, Shu Liangyin, Chen Yifan, Hu Dingli, Tan Yuhua, Yang Sixuan, Liu Lianghong, He Qingjiao, Liu LH., He QJ. |
| Construction of a multidimensional quality evaluation system for Scutellariae Radix: Integrated research on chemical components, biological potency, near-infrared spectroscopy technology, and intelligent algorithms | 10.1016/j.saa.2026.127514 | 2026-01-21 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 4.6 | Guiling Peng, Qianqian Xue, Huixin Liu, Danni Chen, Qiao Liu, Siyao Zhang, Tulin Lu, Yuejun Wu, Xian Zhang, Fangzhou Yin |
| Self-Assembled Nanocomplexes of Oligomerized Catechins and Deoxyribonuclease-I for Synergistically Enhancing Diabetic Wound Healing | 10.1002/agt2.70235 | 2026-01-13 | Aggregate | 13.7 | Yue Li, Yuye Yang, Huiying Zhang, Rongshuang Xin, Xinyi Pang, Tianxin Li, Wei Liu, Xin Zhou, Zinuo Zhang, Sailong Wang, Xinwei Miao, Jie Dong, Yan Zheng, Zhigui Su, Jun Chen, Mei Dong |
| Topical application of Calvatia lilacina ethanol extract-derived fraction promotes diabetic wound healing | 10.1016/j.fitote.2026.107087 | 2026-01-02 | FITOTERAPIA | 2.6 | Yan Li, Tiantian Dong, Tangtang He, Yurong Zhao, Chenxi Yang, Hanlu Xiao, Ruiwen Mo, Jun Chen, Jie Dong |
| Nanobody Nb07 mitigates sepsis by blocking the PFKM-p53-PD-1 axis to enhance macrophage phagocytosis | 10.7150/thno.124303 | 2026-01-01 | Theranostics | 13.3 | Binbin Ji, Hui Guo, Rong Xing, Miaomiao Sun, Yu Cheng, Chen Yao, Hanyong Zhu, Xuerong Wang, Ruihan Jiang, Xin Chen, Zimeng Liu, Suyan Wang, Fei Xu, Fangyu Zhang, Fuxing Dong, Xiucheng Pan, Jing Yang, Yuchen Pan |
| Multitarget inhibitory effects of lemongrass essential oil on Porphyromonas gingivalis: synergistic regulation of heme utilization, biofilm formation, and the metabolic pathway of ferroptosis | 10.1186/s12906-025-05210-4 | 2025-12-07 | BMC Complementary Medicine and Therapies | 3.4 | Yuan Yue, Sun Jinlong, Raka Rifat Nowshin, Liu Zixuan, Zhang Zhongwei, Xiao Junsong, Wu Hua |
| Expression and Biological Activity of Recombinant Type IV Collagen | 10.1021/acs.jafc.5c02431 | 2025-12-04 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 6.2 | Yingxue Chang, Zhuo Zhang, Weigang Yuwen, Ru Xu, Rongzhan Fu, Chenhui Zhu, Weina Li, Zhiguang Duan, Daidi Fan |
| Synthetic Lethal Therapy Based on Dimorphism for Systemic Infection of Drug-Resistant Candida albicans | 10.1002/advs.202518196 | 2025-12-03 | Advanced Science | 14.1 | Yang Gao, Jiahe Su, Jiawei Yuan, Qinyan Cao, Yue Wu, Yuyang Xiao, Guang Yang, Ruomu Xia, Jingpeng Yang, Yanan Li, Lina Wu, He Huang, Lingtong Meng |
| Hepatic soluble epoxide hydrolase mediates osteoclastogenesis by suppressing the Nrf2 signaling pathway: a novel mechanism of redox imbalance in osteoporosis | 10.1016/j.freeradbiomed.2025.11.036 | 2025-11-19 | FREE RADICAL BIOLOGY AND MEDICINE | 8.2 | Bo Liu, Xiu Yang, Hongli Chang, Peipei Li, Hongguang Chang, Xuenan Wang, Ji-chun Zhang, Wubing He |
| Bilayer Biomimetic Scaffolds Loaded with Mesenchymal Stem Cell Secretomes Promote Diabetic Wound Healing | 10.3390/gels11110845 | 2025-10-22 | Gels | 5.3 | Fangling Shen, Yiting Chen, Hongwen Li, Qi Zhang, Qixiong Ji, Linyuan Zou, Zhe Wang, Zhengyao Wu, Shengkai Yu, Hua Zhang, Qin Song |
| Ketogenic diet inhibits glioma progression by promoting gut microbiota-derived butyrate production | 10.1016/j.ccell.2025.09.002 | 2025-09-25 | CANCER CELL | 44.5 | Ming-Liang Chen, Ying He, Xun-Hu Dong, Hao-Fei Liu, Ze-Xuan Yan, Xiao-Lu Lu, Qing-Qing Miao, Qing-Ning Zhao, Hang Zhang, Li Luo, Shuai Wang, Jing-Yuan Li, Dong-Fang Xiang, Yong Lin, Tian-Ran Li, Xin-Yue Zhou, Yang-Yang Zhou, Min Mao, Xia Zhang, Hong Wei, Yu Shi, Xin-Dong Liu, Yi-Fang Ping, Xiu-Wu Bian |
| Structural elucidation, gut fermentation, and immunomodulatory mechanisms of β-glucan polymorphs | 10.1016/j.ijbiomac.2025.146940 | 2025-08-20 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.5 | Rui Huo, Sarina Ma, Ying Miao, Minjun Sun, Rui Wang, Dongyan Li, Tong Jia, Meili Zhang |
| Acetylcholine Suppression by P. gingivalis Extracellular Vesicles Drives Osteoclastogenesis and Bone Loss via the Cyp4f40 | 10.1096/fj.202500112RR | 2025-08-19 | FASEB JOURNAL | 4.2 | Xuanyu Chen, Jiyuan Zou, Zhikang Su, Ping Liang, Yuxin Ye, Zhiyuan Ye, Jiang Li, Li Yang, Lvhua Guo, Tao Luo |
| Platelet-Derived Extracellular Vesicles Alleviate Psoriatic Inflammation via Mitochondrial Transfer to Macrophages | 10.1111/exd.70152 | 2025-08-17 | EXPERIMENTAL DERMATOLOGY | 3.1 | Bing Wang, Dandan Li, Chaolan Pan, Yumeng Wang, Yidong Tan, Yijun Yang, Haifei Liu, Zhirong Yao, Hui Zhang, Zhicheng Wang |
| Unraveling the characteristic structures of Cuscuta chinensis Lam. polysaccharides and its inhibitory effects on non-alcoholic fatty liver disease | 10.1016/j.ijbiomac.2025.146798 | 2025-08-12 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.5 | Zhuqian Xiao, Huilin Zheng, Siyao Zhang, Qin Li, Yurui Wang, Hongpeng Wang, Tingwei Wang |
| Empagliflozin targeted the immune-related gene PIK3CA in type 2 diabetes mellitus treatment: network pharmacology analysis and experimental evidence | 10.1186/s13098-025-01895-2 | 2025-08-07 | Diabetology & Metabolic Syndrome | 3.9 | Zhong Heng, Sun Guo-Juan, Du Fu-Man, Wang Wei-Min, Duan Bin-Hong, Qiao Hong |
| CSF1R inhibitor (PLX3397) alleviates experimental periodontitis by reducing macrophage senescence through the PI3K/AKT/FOXO1 signaling pathway | 10.1038/s41598-025-13153-6 | 2025-07-28 | Scientific Reports | 3.9 | Kang Jiabing, Zhan Jifan, Yuan Yiting, Wei Yan, Xiao Tianjiao, Li Li, Fang Hui, Tian Ai |
| Investigation of differential Multi-Mode antibacterial mechanisms of essential oils of Satureja montana L. and Leptospermum scoparium J.R.Forst. & G.Forst. Against Porphyromonas gingivalis | 10.1186/s12906-025-05007-5 | 2025-07-22 | BMC Complementary Medicine and Therapies | 3.4 | Yue Yuan, Xiuli Hui, Zixuan Liu, Jinlong Sun, Rifat Nowshin Raka, Junsong Xiao, Zhongwei Zhang, Hua Wu |
| METTL3 suppresses inflammatory responses by regulating SIRT1-mediated HMGB1 release in Sepsis | 10.1016/j.ejphar.2025.177959 | 2025-07-16 | EUROPEAN JOURNAL OF PHARMACOLOGY | 4.7 | Lijuan Zhang, Fangqing Zuo, Junda Li, Zhuo Zeng, Yajie Chen, Yingying Lan, Yu Chen, Yali Gong, Jing Chen, Gaoxing Luo, Huapei Song, Yizhi Peng, Zhiqiang Yuan |
| Network pharmacology analysis and experimental validation of EGCG's therapeutic effects against acute lung injury in sepsis | 10.1016/j.intimp.2025.115175 | 2025-07-12 | INTERNATIONAL IMMUNOPHARMACOLOGY | 4.7 | Xianwei Jin, Qiaoling Weng, Kejian Qian, Fen Liu |
| Engineering Injectable, Adhesive, and Drug-Free Hydrogel for Efficient Acute Otitis Media Treatment by Biofilm Disruption and Immune Modulation | 10.1002/adhm.202502562 | 2025-07-06 | Advanced Healthcare Materials | 9.6 | Qingjun Jiang, Yuqi Huang, Baoying Xu, Dehong Yu, Yu Chen, Xueling Wang |
| A Multifunctional MXene@CeO2-Enhanced Hydrogel Dressing for Synergistic Photothermal Antibacterial and Antioxidative Therapy in Wound Healing | 10.1002/adhm.202500656 | 2025-06-26 | Advanced Healthcare Materials | 9.6 | Qianyu Guo, Miaomiao Zhang, Jinyao Zheng, Zongjia Li, Yuanhao Zhang, Ying Chen, Yu Chen, Xiue Jiang, Jilin Tang |
| Injectable hyaluronic acid hydrogel for corneal neovascularization management by inhibiting oxidative stress, inflammation and neovascularization | 10.1016/j.matdes.2025.114248 | 2025-06-15 | MATERIALS & DESIGN | 7.9 | Yingying Wei, Jiahao Xu, Xu Yang, Qing Wang, Linyu Long, Daihua Fu, Fanjun Zhang, Shibo Tang, Yunbing Wang |
| A prebiotic inulin derivative-containing liposome for oral berberine delivery improves the orthotopic colorectal cancer chemotherapy | 10.1039/D5NR01337B | 2025-06-12 | Nanoscale | 5.1 | Anqi Xia, Wenhui Yuan, Shanshan Xu, Ting Wu, Wenlu Yan, Ying Cao, Yaping Li, Qi Yin |
| Dehydrodiisoeugenol targets NOD2 exerting dual effects against colitis and colorectal cancer: a double-edged sword | 10.1186/s10020-025-01193-7 | 2025-06-05 | MOLECULAR MEDICINE | 6.4 | Yi Feiyang, Chen Nianzhi, Zhao Maoyuan, Gu Zhili, Yuan Yun, Tang Xuegui, Liu Fang |
| Pexidartinib Inhibits Macrophage Senescence Through Glycolysis in Periodontitis Microenvironment | 10.1016/j.identj.2025.100843 | 2025-06-02 | INTERNATIONAL DENTAL JOURNAL | 3.7 | Jifan Zhan, Jiabing Kang, Yan Wei, Tianjiao Xiao, Hui Fang, Li Li, Yiting Yuan, Yongchun Zhang, Jie Zhang, Ai Tian |
| Evaluation of the nutritional, in vitro protein digestive and bioactive characteristics of a quinoa-based protein beverage | 10.1016/j.fochx.2025.102609 | 2025-05-31 | Food Chemistry-X | 8.2 | Mengzhuo Li, ManLi Zhu, Junjuan Wang, Liang Zou, Xiaoyan Zhao, Dan Wang, Yuanyan Zhao, Zhiwen Ge, Lizhen Zhang, Peiyou Qin |
| Discovery of Novel Potent Molecule for Treating Cisplatin-Induced Acute Kidney Injury Driven by Phenotypic Screening | 10.1021/acs.jmedchem.5c00954 | 2025-05-31 | JOURNAL OF MEDICINAL CHEMISTRY | 6.8 | Yan Lou, Feilong Zhou, Daxing Shi, Yu Zhang, Jin Dong, Xiaobao Shen, Xing Chen, Xinhua Liu |
| Development of a highly effective anti-aging and soothing recombinant Col III | 10.1007/s42452-025-07081-3 | 2025-05-21 | Discover Applied Sciences | 0 | Wang Yuefeng, Zhang Jing, Xu Guoxia, Wang Ruiyan, Zhou Wei |
| Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasis | 10.1038/s41467-025-59579-4 | 2025-05-13 | Nature Communications | 15.7 | Yang Xiao, Rong Kewei, Fu Shaotian, Yang Yangzi, Liu Shasha, Zhang Chenyu, Xu Kang, Zhang Kai, Zhu Yingchun, Hao Yongqiang, Zhao Jie, Fu Jingke |
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| Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria | 10.1021/acs.jafc.5c01087 | 2025-04-24 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 6.2 | Shen Li, Yuan Liu, Zixi Gao, Chao An, Hui Gu, Heng Yin, Rongzhan Fu, Liang Shi, Wenjiao Xue, Daidi Fan, Qiang Fei |
| Cardiomyocyte-derived Galectin-9 induces macrophage M2 polarization via the TIM3 pathway to attenuate myocardial remodeling post-myocardial infarction | 10.1007/s11010-025-05277-0 | 2025-04-22 | MOLECULAR AND CELLULAR BIOCHEMISTRY | 3.7 | Huang Jiabing, Hu Weitong, Xiong Hongliang, Zhou Yue, Cao Fangying, Ding Congcong, Li Yunde, Chen Mingxian |
| PGC-1α mediates migrasome secretion accelerating macrophage–myofibroblast transition and contributing to sepsis-associated pulmonary fibrosis | 10.1038/s12276-025-01426-z | 2025-04-01 | EXPERIMENTAL AND MOLECULAR MEDICINE | 12.9 | Peng Yawen, Mei Shuya, Qi Xiaohui, Tang Ri, Yang Wenyu, Feng Jinhua, Zhou Yang, Huang Xi, Qian Guojun, Xing Shunpeng, Gao Yuan, Xu Qiaoyi, He Zhengyu |
| A self-assembling peptide-based hydrogel containing NF-κB inhibitors and NGF for peripheral nerve injury repair | 10.1088/1758-5090/adc340 | 2025-03-27 | Biofabrication | 8 | HaiTao Su, QiuPing Ye, Di Wang, AnQi Liu, YaGuang Wang, Ye Zhang, LiJun Weng |
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| An anti-inflammatory and anti-fibrotic Janus hydrogel for preventing postoperative peritoneal adhesion | 10.1016/j.mtbio.2025.101637 | 2025-03-05 | Materials Today Bio | 10.2 | Zhengjun Li, Lili Yang, Qi Jin, Wen Li, Yue Li, Yan Zheng, Mei Dong, Yaoyao Bian |
| Effects of Surface Charge of Inhaled Liposomes on Drug Efficacy and Biocompatibility | 10.3390/pharmaceutics17030329 | 2025-03-03 | Pharmaceutics | 5.5 | Jinniu Zhang, Yun Huang, Wenhao Shen, Yixing Zeng, Yingjing Miao, Nianping Feng, Tianyuan Ci |
| Anti-inflammatory effect of polysaccharides from Sambucus williamsii Hance roots in lipopolysaccharide-stimulated RAW264.7 macrophages and acute lung injury in mice | 10.1016/j.ijbiomac.2025.141368 | 2025-02-21 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 8.5 | Xianqun Meng, Qing Wei, Siyi Wang, Shanshan Liang, Dan Wang, Haixue Kuang, Qiuhong Wang, Tian Xie |
| β-Sitosterol inhibits osteoclast activity and reduces ovariectomy-induced bone loss by regulating the cAMP and NF-κB signaling pathways | 10.1016/j.cellsig.2025.111672 | 2025-02-19 | CELLULAR SIGNALLING | 3.7 | Huizhi Guo, Xiaojun Chen, Jinglan Li, Guoye Mo, Yongxian Li, Yongchao Tang, Yuan Kai, Shuncong Zhang |
| Golden Tandem of Photothermal Ablation and Simultaneous Anti-Inflammation in One Nanoparticle for Activated Macrophage-Targeted Atherosclerosis Treatment | 10.2147/IJN.S503774 | 2025-02-07 | International Journal of Nanomedicine | 6.5 | Yuqing Lu, Yan Wang, Yize Li, Yunan Li, Yao-Wen Jiang, Jingjing Li |
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| Intratracheal delivery of macrophage targeted Celastrol-loaded PLGA nanoparticles for enhanced anti-inflammatory efficacy in acute lung injury mice | 10.1016/j.ejpb.2024.114511 | 2024-09-21 | EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS | 4.4 | Yinlian Yao, Shilong Fan, Yinqiang Fan, Xin Shen, Xingxing Chai, Yiming Shao, Jiang Pi, Xueqin Huang, Yiming Shao, Zhikun Zhou, Yue Zhao, Hua Jin |
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| CD8+ T-cell deficiency protects mice from abdominal aortic aneurysm formation in response to calcium chloride2 | 10.1097/HJH.0000000000003823 | 2024-08-14 | JOURNAL OF HYPERTENSION | 3.3 | Lin Zhuo, Zhao Mantong, Zhang Xian, Piao Jinshun, Zheng Xintong, Shu Shangzhi, Zhao Longguo, Zhang Meiping, Shi Guo-Ping, Lei Yanna, Cui Rihua, Yue Xueling, Cheng Xian Wu |
| Oral delivery of pH-sensitive nanoparticles loaded Celastrol targeting the inflammatory colons to treat ulcerative colitis | 10.1177/20417314241265892 | 2024-08-10 | Journal of Tissue Engineering | 6.7 | Yue Zhao, Yinlian Yao, Shilong Fan, Xin Shen, Xingxing Chai, Zimin Li, Jiachun Zeng, Jiang Pi, Zhikun Zhou, Gonghua Huang, Hua Jin |
| Muti-interactions based 3D-printed osteochondral biomimetic scaffold for BMP-2 sustained release | 10.1016/j.eurpolymj.2024.113343 | 2024-07-26 | EUROPEAN POLYMER JOURNAL | 5.8 | Zhonglian Wu, Haidi Sun, Xu Hu, Jian Yang, Zehao Gu, Chirun Wang, Hui Chong, Jihang Dai, Hang Yao, Dong-An Wang |
| Adipose-derived stem cell exosomes loaded with icariin alleviates rheumatoid arthritis by modulating macrophage polarization in rats | 10.1186/s12951-024-02711-1 | 2024-07-18 | JOURNAL OF NANOBIOTECHNOLOGY | 10.6 | Yan Qiqi, Liu Haixia, Sun Shiyue, Yang Yongsheng, Fan DanPing, Yang Yuqin, Zhao Yukun, Song Zhiqian, Chen Yanjing, Zhu Ruyuan, Zhang Zhiguo |
| Melatonin alleviates high-fat-diet-induced dry eye by regulating macrophage polarization via IFT27 and lowering ERK/JNK phosphorylation | 10.1016/j.isci.2024.110367 | 2024-06-24 | iScience | 4.6 | Yuying Cai, Xin Zhang, Chuanxi Yang, Yaping Jiang, Yihui Chen |
| Development of an injectable oxidized dextran/gelatin hydrogel capable of promoting the healing of alkali burn-associated corneal wounds | 10.1016/j.ijbiomac.2024.132740 | 2024-05-31 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 7.7 | Zhengwei Ge, Linyu Long, Fanjun Zhang, Ruiqi Dong, Zhongping Chen, Shibo Tang, Li Yang, Yunbing Wang |
| Activation of the cGAS-STING pathway by viral dsDNA leading to M1 polarization of macrophages mediates antiviral activity against hepatitis B virus | 10.1016/j.imbio.2024.152810 | 2024-05-17 | IMMUNOBIOLOGY | 2.5 | Qiyin Zong, Hao Zhang, Futing Liu, Jianfei Li, Qian Liu, Zhi Duan, Wanlu Duan, Mengqi Ruan, Jingjing Zhang, Yan Liu, Qiang Zhou, Qin Wang |
| Polyethylene Glycol-Modified Cationic Liposome as a Promising Nano Spray for Acute Pneumonia Treatment | 10.3390/polym16101384 | 2024-05-12 | Polymers | 4.7 | Kai Wang, Dagui Chen, Chenxi Zhang, Lu Lu, Fusheng Shang, Yinghua Li |
| Total iridoid glycoside extract of Lamiophlomis rotata (Benth) Kudo accelerates diabetic wound healing by the NRF2/COX2 axis | 10.1186/s13020-024-00921-1 | 2024-03-22 | Chinese Medicine | 5.3 | Geng Xiaoyu, Wang Ying, Li Huan, Song Liang, Luo Chen, Gu Xiaojie, Zhong Haixin, Chen Huilin, Chen Xinzhu, Wang Jianwei, Pan Zheng |
相关实验
RAW264.7因是单核巨噬细胞其生物学特性就是贴壁特别牢，普通的方法根本就不可能将它消化下来，这也是养这种细胞最头痛的问题。现将我的一点心得，简介如下：消化液：0.5％胰酶、0.2％EDTA，实验前加温到37度以50ml培养瓶为例：1、细胞贴壁生长，长满瓶底80％时，弃去培养液，加D-HANKS 漂洗 两次；2、 加入含胰酶和EDTA的消化液1－2ml,消化37℃约2-5min，镜下可见细胞基本圆形回缩即中止消化，弃消化液加D-HANKS 漂洗两次；3、加入新培养液10ml复吹打混悬细胞
单核/巨噬细胞的分化成熟及组织分布 单核／巨噬细胞均来源于骨髓干细胞。发育成单核细胞后，进入血流分布于各种器官组织，并有不同的命名。如图2-12 单核巨噬细胞的组织分布 成熟的单核吞噬细胞表达多种表面分子．其中受体多达数十种，如免疫球蛋白的Fc受体FcyRI(CD64)、FcγRⅡ(CD32)、FcγRⅢ(CDl6)，补体受体CRl和CR3和各种模式识别受体(包括甘露糖受体、清道夫受体、TLR)，以及多种细胞因子、激素、神经肽、多糖、糖蛋白和脂蛋白的受体(图2―13)，介导单核／
・ Lymphocyte Transformation (Donis-Keller lab) Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque
文献支持
小鼠单核巨噬细胞白血病细胞；RAW264.7(STR)
￥1200 - 1500
