- ￥1350 - 2400
- Solarbio|ActivAb
- 北京
- K011390P
- 2025年08月19日
- WB;IF;IHC
- Rabbit
- Mouse;Human;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of human GPX4
- 亚型：
IgG
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Polyclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Mouse;Human;Rat
- 抗原来源：
A synthetic peptide of human GPX4
- 目录编号：
2879
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;IF;IHC
- 浓度：
WB 1:500-1000. IHC 1:50-100. IF 1:50-200.
- 靶点：
Glutathione Peroxidase 4
- 抗体英文名：
Anti-Glutathione Peroxidase 4 Polyclonal Antibody
- 抗体名：
谷胱甘肽过氧化酶4抗体
- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2400.0 |
| 基本信息 | |
| 英文名称 | Anti-Glutathione Peroxidase 4 Polyclonal Antibody |
| 中文名称 | 谷胱甘肽过氧化酶4抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Mouse;Human;Rat |
| 应用 | WB;IF;IHC |
| 稀释比例 | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. |
| 密度 | |
| 靶标 | Glutathione Peroxidase 4 |
| 别名 | Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase |
| 背景说明 | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a moonlighting protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. |
| Swiss Prot | P36969 |
| Gene ID | 2879 |
| 亚细胞定位 | Cytoplasm Mitochondrion |
| 免疫原 | A synthetic peptide of human GPX4 |
| 理论分子量 | 22kDa |
| 实际分子量 | 17kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | Liquid in PBS containing 50% glycerol，150mM NaCl and 0.02% sodium azide，pH 7.4. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验The Peroxidase-Antiperoxidase (PAP) Method
-antibody solution, the PAP immune complex. The horseradish peroxidase enzyme, itself an immunogenic protein, is used to inoculate a given species, and a polyclonal immune response is generated against the enzyme. This antiserum is harvested and placed
Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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