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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D4-2,4,4'-Trichlorobiphenyl
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1219799-32-2;unlabelled:7012-37-5
- 规格:
10mg///100mg///200mg
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文献和实验or 4°C; Wash 3 times in 1X PBS for 5 minutes each; Permeabilize with freshly made 1X PBS/0.5% Triton X-100 (containing the RNAse-inhibitor, 2mM Vanadyl Ribonucleoside Complex, in case of a subsequent RNA-FISH) on ice for 3.5-5 minutes
, you will use 1-5 µg per lane. 2. Harvest the worms before the plates starve. After the bacteria are gone, the worms begin breaking down the agarose and releasing enzyme inhibitors. It is best to harvest when the worms are forming a wave across the plate
safety precautions - If you store it frozen, take the alpha 32P-dATP out of the freezer to thaw. - Put 50 ng DNA to be labelled in a total volume of 18.9 µl H20. This is usually 1-4 µl of a typical cosmid prep. It is unnecessary to cut cosmid DNA
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