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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
(2s,4s)-4-cyclohexylpyrrolidine-2-carboxylic acid
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
130092-20-5
- 规格:
10mg///100mg///200mg
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
Abstract Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described. Introduction
Methods for the Detection of D-Amino-Acid Oxidase
phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham
products by high-pressure liquid chromatography (HPLC). At this point, we also present a complete summary in Table 1 comparing the various approaches used for the measurements of arachidonic acid metabolites derived from the prostaglandin endoperoxide
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