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| 规格: | 10ml | 产品价格: | ¥1800.0 |
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文献和实验Single Quantum Dot Tracking of Membrane Receptors
fluorophores and fluorescent proteins rapidly photobleach, whereas gold particles and latex beads, although more stable, are bulky and usually stain only one protein per experiment. In comparison, QDs are bright and photostable fluorescent probes with a size
Particles)的漂洗1)取三管链霉抗生物素蛋白-磁珠,轻弹离心管的底部使SA-PMPs重悬直至其充分分散,吸取悬液合并于一管。2)使汇总的SA-PMPs重悬直至其充分分散,然后将离心管置于磁柱中直至SA-PMPs已经聚集在离心管壁上(大约30秒)。小心地移出上清。不要离心沉淀颗粒。3)用0.5%26times SSC漂洗SA-PMPs三次(每次0.9ml),每次都使用磁柱来捕获它们,然后小心地移去上清。4)重悬漂洗过的SA-PMPs于0.3ml的0.5%26times SSC中。4. Oligo(dT
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
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