- ￥1800 - 3000
- 百欧泰
- ABQD-0050-Biotin
- 2025年08月18日
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- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10ml/20ml
| 规格： | 10ml | 产品价格： | ￥1800.0 |
|---|---|---|---|
| 规格： | 20ml | 产品价格： | ￥3000.0 |
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文献和实验DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
. Before stopping the reaction, check the nick-translation reaction by running 1/10 of the reaction on a 1% agarose gel. One should obtain a smear of biotin labeled fragments ranging from 500 to 200 nt. Stop the reaction according manufacturer instructions.
FLUORESCENT STAINING OF CELLS Fluorescent Phalloidin Staining Materials 1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2. PBS
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