- ￥706 - 3699
- DIMA BIOTECH/缔码生物
- 武汉
- PME101805
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant human IDE Protein with C-terminal 10×His tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥706.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2500.0 |
| 规格： | 100μg | 产品价格： | ￥3699.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | IDE |
| Uniprot ID | P14735 |
| 别名 | INSULYSIN |
| 背景 | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009] |
| 产品英文名称 | Recombinant human IDE Protein with C-terminal 10×His tag |
| 分子结构 | IDE(Met1-Leu1019) 10×His tag |
| 分子量 | The protein has a predicted molecular mass of 119.3 kDa after removal of the signal peptide. |
| 纯度 | The purity of the protein is greater than 80% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | C-10×His tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human IDE Protein, His Tag on SDS-PAGE under reducing condition.
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文献和实验.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
-S10 柱子对于去污剂有高度的结合容量，比如每毫升固定的树脂可以结合 6 mg SDS 和 14 mg Triton® X-100。Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10. ag 蛋白的纯化 带有融合标签的表达产物----融合蛋白的纯化通常也是紧接细胞破碎工作进行的。设计融合标签的主要目的之一就是为了方便
性定量检测等微型化、连续化， 尽量减少空间， 加快速度， 实现生物分析系统的芯片化. 这些分析过程中的某一步或几步微型化集成到一块芯片上就能获得具有特殊功能的芯片， 如用于样品制备的针对DNA 分析的介电电泳芯片[ 1 ]； 用于基因突变检测、基因表达和测序的DNA 微阵列芯片[ 2 ]和肽核酸(pep t ide nucleic acids， PNA ) 芯片[ 3， 4 ]； 用于蛋白质分析的蛋白质芯片[ 5 ]； 研究蛋白质与DNA 互作的双链DNA 芯片[ 6 ]等. 把上述分析过程全部或部分
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