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- 百欧泰
- ABQD-5000
- 2025年08月14日
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- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10mg/ml
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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
Millipore filter, 0.45 um 1 Millipore #UFC3OHVNB . *50X dNTPs stock Final conc. dATP (100 mM) 25 uL 25 mM dCTP
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
and cycle as usual using the terminator program as described by the manufacturer (i.e. preheat at 96oC followed by 25 cycles of 96℃ for 15 seconds, 50oC for 1 second, 60oC for 4 minutes, and then link to a 4oC hold). 4. Proceed with the spin column
Preparation of Fluorescent DNA Probe from mRNA: Yeast
DNA or RNA (10 mg/mL in TE) 1.0 uL 20X SSC 3.0 uL Millipore filter, 0.45 um 1 Millipore #UFC3OHVNB . *50X dNTPs stock
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