- ￥480 - 1600
- Solarbio|ActivAb
- 北京
- K112758P
- 2025年08月13日
- WB;IHC
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of Human PHKA1/2
- 亚型：
IgG
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Polyclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Human;Mouse;Rat
- 抗原来源：
A synthetic peptide of Human PHKA1/2
- 目录编号：
5255/5256
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;IHC
- 浓度：
WB 1:2000-5000. IHC 1:25-100.
- 靶点：
PHKA1/2
- 抗体英文名：
Anti-PHKA1/2 Polyclonal Antibody
- 规格：
20ul/100ul/50ul
| 规格： | 20ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
| 规格： | 50ul | 产品价格： | ￥960.0 |
| 基本信息 | |
| 英文名称 | Anti-PHKA1/2 Polyclonal Antibody |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Human;Mouse;Rat |
| 预测交叉反应 | Rabbit(100%);Fish(100%) |
| 应用 | WB;IHC |
| 稀释比例 | WB 1:2000-5000. IHC 1:25-100. |
| 密度 | |
| 靶标 | PHKA1/2 |
| 别名 | 5330411D17; 9830108K24Rik; kinase PHKA1; KPB1; KPB1_HUMAN; MGC132604; Pcyt1b; PHKA; PHKA1; Phosphorylase b kinase regulatory subunit alpha; Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform; Phosphorylase kinase alpha M subunit; phosphorylase kinase, alpha 1 (muscle); RP23 210E20.1; skeletal muscle isoform; dJ499B10.2 (phosphorylase kinase, alpha 2 (liver) (PYK)); GSD9A; PHK; PHKLA; Phosphorylase b kinase alpha regulatory chain, liver isoform; phosphorylase b kinase regulatory subunit alpha, liver isoform; Phosphorylase kinase , liver, alpha-2 subunit; |
| 背景说明 | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1. |
| Swiss Prot | P46020/P46019 |
| Gene ID | 5255/5256 |
| 亚细胞定位 | Cell membrane Cytoplasm |
| 免疫原 | A synthetic peptide of Human PHKA1/2 |
| Public Immunogen Range | 40-90/1223aa |
| 理论分子量 | 129/136/137kDa |
| 实际分子量 | 140kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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