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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of human Histone H3.1
- 亚型:
IgG
- 保存条件:
Store at -20℃. Avoid freeze / thaw cycles.
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,Solar Fluor 350,Solar Fluor 405,Solar Fluor 488,Solar Fluor 532,Solar Fluor 555,Solar Fluor 568,Solar Fluor 594,Solar Fluor 633,Solar Fluor 640,Solar Fluor 647,Solar Fluor 660,Solar Fluor 680,Solar Fluor 750
- 适应物种:
Human;Mouse;Rat;Monkey
- 抗原来源:
Recombinant protein of human Histone H3.1
- 目录编号:
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
- 级别:
实验级别
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB;IHC
- 浓度:
WB 1:8000-12000. IHC 1:25-100.
- 靶点:
Histone H3.1
- 抗体英文名:
Anti-Histone H3.1 Polyclonal Antibody
- 抗体名:
组蛋白 H3.1抗体
- 规格:
100ul/50ul
| 规格: | 100ul | 产品价格: | ¥780.0 |
|---|---|---|---|
| 规格: | 50ul | 产品价格: | ¥480.0 |
| 基本信息 | |
| 英文名称 | Anti-H3C1 Polyclonal Antibody |
| 中文名称 | 组蛋白 H3.1抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Mouse;Human |
| 应用 | WB;IHC |
| 稀释比例 | WB 1:8000-12000. IHC 1:25-100. |
| 密度 | |
| 靶标 | H3C1 |
| 别名 | H3C1, H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J |
| 背景说明 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| Swiss Prot | P68431 |
| Gene ID | 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 |
| 亚细胞定位 | Nucleus |
| 免疫原 | Recombinant protein of human H3C1 |
| Public Immunogen Range | 1-137aa |
| 理论分子量 | 15kDa |
| 实际分子量 | 18kDa |
| EC | |
| 纯化方法 | Octanoic acid-ammonium sulfate precipitation |
| 储存液 | PBS with 50% Glycerol,0.03% Proclin300,0.5% BSA,pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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