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- 文献和实验
- 技术资料
- 抗体名:
组蛋白 H3抗体
- 抗体英文名:
Anti-Histone H3 Polyclonal Antibody
- 靶点:
Histone H3
- 浓度:
WB 1:8000-12000. IHC-P 1:25-100.
- 应用范围:
WB ; IHC-P
- 宿主:
Rabbit
- 适应物种:
Human;Mouse;Rat;Monkey
- 保质期:
Store at -20℃. Avoid freeze / thaw cycles.
- 抗原来源:
Recombinant protein of human Histone H3.1
- 目录编号:
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
- 级别:
实验级
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,SAlexa Fluor 350,SAlexa Fluor 405,SAlexa Fluor 488,SAlexa Fluor 532,SAlexa Fluor 555,SAlexa Fluor 568,SAlexa Fluor 594,SAlexa Fluor 633,SAlexa Fluor 640,SAlexa Fluor 647,SAlexa Fluor 660,SAlexa Fluor 680,SAlexa Fluor 750
- 保存条件:
Store at -20℃. Avoid freeze / thaw cycles.
- 形态:
干粉/液体
- 亚型:
IgG
- 免疫原:
Recombinant protein of human Histone H3.1
- 规格:
50ul/100ul/100ul(PBS Only)
| 规格: | 50ul | 产品价格: | ¥480.00 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥780.00 |
| 规格: | 100ul(PBS Only) | 产品价格: | ¥1560.00 |
【抗体靶点简介】
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.【实验操作视频参考】
Western blot实验参考操作流程 | ![]() Immunohistochemistry实验参考操作流程 |
抗体在科研领域应用十分广泛,对解析生物学过程、探究疾病发病机制、创新药物研发与临床治疗研究均起到关键支撑作用。
索莱宝现有30000余种经过严格实验验证的抗体产品,全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向,适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体,以及多种标记类型的一抗、二抗,充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台,从免疫制备、纯化提纯到实验验证全流程严格筛选评估,全方位保障每一支抗体的性能与品质。
此外,索莱宝已完善一站式技术服务平台,可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测,以及多克隆/单克隆抗体定制等个性化定制服务,为科研用户提供全方位解决方案。



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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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