- ￥307.80
- AMEKO
- 中国
- AB60912-100mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Cefdinir
- 库存：
99
- 供应商：
鹿森生物
- CAS号：
91832-40-5
- 规格：
100mg
纯度：HPLC≥99%
保存温度：-20℃
英文名称：Cefdinir
分子式：C14H13N5O5S2
HGOMAEKSYQLNWBDFJXZPUIRCVT
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
. Test each tube w/H2O and discard.Add protein mixture to dialysis tube. Stir slowly in 100 mM Tris buffer or Binding buffer until pink color is out.Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume.Dilute the mixture
Antibody Staining of Dissected Gonads
in the dissecting scope it is tedious. Materials PBS -- Sambrook et al. Molecular Cloning book. PBS/0.2 mM Levamisole (from 100 mM Levamisole stock). PBS/0.1% Tween 20 (PBTw) Blocking buffer; 30 % goat serum in PBTw or 1 mg/ml BSA in PBTw.
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