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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP(试剂)
- 供应商:
广州市左克生物
- 规格:
10 μg
产品简介:
品牌:SBI
货号:HR700PA-1
产品名称:Gene Knock-Out HR Targeting Vector w/Dual Selectio(试剂)
规格:10 μg
BSL:~
品牌介绍:
System Biosciences,简称SBI,美国加州湾区新成立的生技公司,致力于独特、创新生物技术之开发,以研发利于基因及蛋白质功能鉴定、研究之崭新方法和工具为宗旨。 现阶段研发重心为RNA干扰(RNAi)研究之相关工具。
System Biosciences (SBI) 致力于开发独特、革新的技术,为客户研究蛋白组学和基因组学功能提供研究工具。SBI 是专业的慢病毒产品公司,提供基于慢病毒的所有相关产品、质粒、试剂盒及相关配套试剂和慢病毒延伸产品如IPS细胞多功能性诱导试剂盒和RNAi筛选文库。
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
1/2) and DNA of gene targeting vector. The nuclease induces a double‐strand break (DSB) at the target site that stimulates homologous recombination (HR) of the vector with the target locus, resulting into a knockin (KI) allele. Manipulated embryos
of up to 800 ng DNA containing the targeting constructsand the HR template vector (or single-stranded DNAoligos, see Note 9 ) in a microcentrifuge tube for each well oftransfection on a 24-well plate. Generally, apply a molar ratio of1:3–5:1 for the targeting
Generate siRNA Populations with Dicer
Figure 2. Specificity of siRNA Cocktails When Targeting a Conserved Region of a Gene Family. HeLa cells were transfected with siRNA cocktails derived from the human STAT3 gene generated using the Silencer ™ siRNA Cocktail Kit (Dicer). 48 hr
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