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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
309-055-006
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-Human IgG, F(ab')2 Fragment Specific
- 规格:
1ml
产品简介:
品牌:Jacksonimmuno
货号:309-055-006
产品名称:Alkaline Phosphatase-conjugated AffiniPure® Rabbit Anti-Human IgG, F(ab')2 Fragment Specific
https://www.jacksonimmuno.com/catalog/products/309-055-006
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
each, with 0.5% BSA in TBST. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection which employs goat anti
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