- ￥1000
- GenScript
- A00613
- 2026年05月27日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州市左克生物
- 库存：
充足
- 保存条件：
4ºC
- 规格：
25 tests
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| Specificity | GenScript THE™ His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide (HHHHHH) coupled to KLH |
| Conjugate | Biotin |
| Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 6G2A9 |
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
Production of Monoclonal Antibody by DNA Immunization with Electroporation
construct was injected intramuscularly with in vivo electroporation into mice, and the specific antibody production in mice was analyzed by enzyme-linked immunosorbent assay (ELISA). Hybridomas were generated by fusing mouse splenocytes with myeloma cells
Immunofluorescent Staining of Mouse and Rat Leukocytes
mAb. It is important to verify that no secondary reagent will bind the BD Fc Block™. Please see description in "The Uses of BD Fc Block™ in Immunophenotyping of Mouse and Rat Leukocytes". For very low-density cell surface markers (e.g.,cytokine
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