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- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- 库存:
充足
- 保存条件:
4ºC
- 规格:
40μg
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| Specificity | GenScript MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags. |
| Host Species | Mouse |
| Immunogen | Purified recombinant MBP fusion protein |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1 |
| Clonality | Monoclonal |
| Clone ID | 5B10B11 |
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文献和实验Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs
diagnosis of both human and animal bacterial and mycoplasmal disease, but similar assays for the diagnostic detection of antigen rather than antibody are not as commonly used. Although many antigen-capture or sandwich ELISAs have been developed
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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