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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- 库存:
充足
- 保存条件:
4ºC
- 规格:
100μg
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| Specificity | THE™ His Tag Antibody [HRP], mAb, Mouse recognizes His tags localized at C-terminal, N-terminal, and internal region of fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Conjugate | Horseradish peroxidase (HRP) |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20°C from date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. Avoid repeated freezing and thawing cycles. |
| Purification | Purified by Protein A affinity chromatograph. |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 6G2A9 |
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
construct was injected intramuscularly with in vivo electroporation into mice, and the specific antibody production in mice was analyzed by enzyme-linked immunosorbent assay (ELISA). Hybridomas were generated by fusing mouse splenocytes with myeloma cells
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Immunofluorescent Staining of Mouse and Rat Leukocytes
mAb. It is important to verify that no secondary reagent will bind the BD Fc Block™. Please see description in "The Uses of BD Fc Block™ in Immunophenotyping of Mouse and Rat Leukocytes". For very low-density cell surface markers (e.g.,cytokine
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