pAdEasy-fiber35

Construction and Characterization of Adenovirus Vectors

), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X

小脑皮质

层，树突的分支繁多，形如侧柏叶状或扇形，铺展在与小脑叶片长轴垂直的平面上。轴突自胞体底部发出，离开皮质进入髓质，终止于小脑内部的核群。 3．颗粒层 此层由密集的颗粒细胞和一些高尔基细胞组成。颗 细胞很小，胞体直径与淋巴细胞近似，有4～5个短树突，树突末端分支如爪状。轴突上行进入分子层呈“T”形分支，与小脑叶片长轴平行，称平行纤维（parallel fiber）。平行纤维穿过一排排蒲肯野细胞的扇形树突，与其树突棘形成突触（图7－35）。一条平行纤维可与400多个蒲肯野细胞建立突触，每个蒲肯野

Characterization of the Picrotoxin Site of GABAA Receptors

Introduction Basic Protocol 1: Saturation Binding of [35S]TBPS to the Picrotoxin Site of GABAA Receptors in Rat Brain Membranes Basic Protocol 2: Competition Assays for [35S]TBPS Binding