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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C; keep this chemical dry and dark.
- 保质期：
见COA
- 库存：
大量
- 供应商：
北京辰辉创聚生物技术有限公司
- CAS号：
N/A
- 规格：
1mg；100mg；1g；Bulk
| 产品信息
品名：NebuChem™ Flurbiprofen D-glucitol ester
货号：NBC-243596
品牌：Nebulabio
规格：1mg；100mg；1g；Bulk
别名：Flurbiprofen D-sorbitol ester
| 产品属性
CAS号：N/A
分子式：C21H25FO7
分子量：408.42
溶解度：DMSO, Methanol
外观：See COA
储存条件：Keep dry, cool and dark.
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Take a 10-20μl aliquot and dilute with 400μl buffer.Read OD at 556 nm and calculate dye concentration based on the molar extinction coefficient of 50,000.D/P should be around 0.75 per tubulin monomer.
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
ratio.Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000} 8.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Drop freeze in liquid N2 after dissolving 2 mg sucrose
Measurement of Phospholipase D Activity
for phorbol ester tumor promoters (reviewed in ref. 1 ). However, with continued study it became apparent that hydrolysis of the polyphosphoinositides by phosphoinositide-specific phospholipase C was not the only pathway through which DAG could be generated
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