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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C; keep this chemical dry and dark.
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- CAS号:
215868-36-3
- 规格:
1mg;100mg;1g;Bulk

| 产品信息
品名:NebuChem™ 6,8-Difluoro-4-methylumbelliferyl β-D-glucuronide
货号:NBC-243327
品牌:Nebulabio
规格:1mg;100mg;1g;Bulk
别名:6,8-Difluoro-4-methyl-2-oxo-2H-1-benzopyran-7-yl β-D-glucopyranosiduronic acid
| 产品属性
CAS号:215868-36-3
分子式:C16H14F2O9
分子量:388.27
溶解度:DMSO, H2O
外观:See COA
储存条件:Keep dry, cool and dark.
For Research Use Only!
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文献和实验Structural Determination of O-Glycans by Tandem Mass Spectrometry
in the regiospecific synthesis of sialylated carbohydrates and glycoconjugates. Recently, the authors described a newly developed nonradioactive screening test for monitoring TS activity (1 ). In this highly sensitive and specific assay, 4-methylumbelliferyl-β-D
(=10 mM) 4.08 mg 4MUG Make Extraction Buffer, Assay Buffer fresh and keep on ice 4MUG = 4-methylumbelliferyl b-D-glucuronide Also prepare: Kontes homogenisers (for eppen tubes) 4 eppen tubes per sample 1:5 Bradford
2. 染色步骤 (1)染色:加入适量配制好的GUS 染液于24孔板的孔中,将待测样品浸到GUS染液中,将24孔板置于37℃保温箱中放置6 h。 (2)漂洗:先后用50%,70%,100%的乙醇漂洗样品,每次浸泡5分钟。 (3)脱色:加入100%乙醇浸泡直至完全脱色。 (4)记录:在体视显微镜下拍照记录。 二、GUS报导基因的定量检测 GUS 能与底物MUG(分子量352.3,4-甲基伞型酮-β-葡萄糖醛酸苷,4-methylumbelliferylβ-D-glucuronide
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