CAS#20689-02-5 | Benzyl 2,3:5,

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

in a yield of 40% after P (III) oxidation and purification. Complete removal of the nucleobases, phosphates, and 2??O ?tert ?butyldimethylsilyl protecting groups leads to the desired propargylated c?di?GMP diester. Cycloaddition of a biotinylated azide

Quantification of Allosteric Interactions at G Protein–Coupled Receptors Using Radioligand Binding Assays

of the same data. Note the reduction in slope (1/ K App ) with no significant change in B max . (C ) Nonlinear regression analysis of the data using GraphPad PRISM, according to Equation followed by Equation . (D ) Nonlinear regression analysis of the data using

HPLC培训教程

，还与Vs、Vm有关。由于t'R、t0较Vs、Vm易于测定，所以容量因子比分配系数应用更广泛。 Ø选择性因子(selectivity factor，α)――相邻两组分的分配系数或容量因子之比。α＝＝  （设k2＞k1）。因k＝t'R/t0，则α＝，所以α又称为相对保留时间（《美国药典》）。 要使两组分得到分离，必须使α≠1。α与化合物在固定相和流动相中的分配性质、柱温有关，与柱尺寸、流速、填充情况无关。从本质上来说，α的大小表示两组分在两相间的平衡分配热力学性质的差异，即分子间相互