CAS#488-82-4 (unlabeled) | D-[

Measuring Receptor Target Coverage: A Radioligand Competition Binding Protocol for Assessing the Association and Dissociation Rates of Unlabeled Compo

. The kinetics of action of acetylcholine antagonists in smooth muscle. Proc. R. Soc. Lond. B Biol. Sci. 164:488‐510.    Smith, D.A., Jones, B.C., and Walker, D.K. 1996. Design of drugs

细胞自噬的研究方法介绍

上清液；每孔加 DMSO(Dimethyl sulfoxide)150 uL，室温下振荡 10 min，立刻在酶联免疫监测仪上测定各孔吸光度值 (选择波长 490 nm)。 实验分组： A 正常培养组 B 0.0001% A 药物培养液 C 0.001% A 药物培养液 D 0.01% A 药物培养液 E 0.1% A 药物培养液 结果分析：细胞存活率% =（加药细胞 OD -本地 OD /对照细胞 OD-本地 OD）×100％ 注：本底 OD 值（完全

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

+ 1 mM MgATP + 10 µM taxol. 3. Transfer flow cell to microscope and setup DIC imaging. 6. Flow in 30 µl 10 ug/ml taxol-stabilized microtubules (unlabeled) sheared to ~5-10 µm length. The taxol-stabilized microtubules are diluted from a 1