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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
OEpiCM (Ovarian Epithelial Cell Medium)
- 规格:
500ml/T
|
货号 |
7311 |
|
产地 |
美国 |
|
缩写 |
OEpiCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Ovarian Epithelial Cell Medium (OEpiCM) is a complete medium designed for optimal growth of normal human ovarian epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human ovarian epithelial cells in vitro.
1.) Friedman E. (2013) "Mirk/dyrk1B kinase in ovarian cancer." Int J Mol Sci. 14: 5560
2.) Cha Y, Kim DK, Hyun J, Kim SJ, Park KS. (2013) " TCEA3 binds to TGF-beta receptor I and induces Smad-independent, JNK-dependent apoptosis in ovarian cancer cells." Cellular Signal. 25: 1245-51.
3.) Takahashi Y, Koyanagi T, Suzuki Y, Saga Y, Kanomata N, Moriya T, Suzuki M, Sato Y. (2012) "Vasohibin-2 expressed in human serous ovarian adenocarcinoma accelerates tumor growth by promoting angiogenesis." Mol Cancer Res. 10: 1135-46.
4.) Semaan A, Qazi AM, Seward S, Chamala S, Bryant CS, Kumar S, Morris R, Steffes CP, Bouwman DL, Mukarah AR, Weaver DW, Gruber SA, Batchu RB. (2011) "MicroRNA-101 Inhibits Growth of Epithelial Ovarian Cancer by Relieving Chromatin-Mediated Transcriptional Repression of p21waf1/cip1." Pharm Res. 28: 3079-90.
5.) Guo AM, Liu X, Al-Wahab Z, Maddippati KR, Ali-Fehmi R, Scicli AG, Munkarah AR. (2011) "Role of 12-lipoxygenase in regulation of ovarian cancer cell proliferation and survival." Cancer ChemotherPharmacol.68: 1273-83.
6.) Selvendiran K, Ahmed S, Dayton A, Kuppusamy ML, Tazi M, Bratasz A, Tong L, Rivera BK, Kalai T, Hideg K, Kuppusamy P. (2010) "Safe and targeted anticancer efficacy of a novel class of antioxidant-conjugated difluorodiarylidenylpiperidones: differential cytotoxicity in healthy and cancer cells." Free RadicBiol Med.48: 1228-35.
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文献和实验
1.) Friedman E. (2013) "Mirk/dyrk1B kinase in ovarian cancer." Int J Mol Sci. 14: 5560
2.) Cha Y, Kim DK, Hyun J, Kim SJ, Park KS. (2013) " TCEA3 binds to TGF-beta receptor I and induces Smad-independent, JNK-dependent apoptosis in ovarian cancer cells." Cellular Signal. 25: 1245-51.
3.) Takahashi Y, Koyanagi T, Suzuki Y, Saga Y, Kanomata N, Moriya T, Suzuki M, Sato Y. (2012) "Vasohibin-2 expressed in human serous ovarian adenocarcinoma accelerates tumor growth by promoting angiogenesis." Mol Cancer Res. 10: 1135-46.
4.) Semaan A, Qazi AM, Seward S, Chamala S, Bryant CS, Kumar S, Morris R, Steffes CP, Bouwman DL, Mukarah AR, Weaver DW, Gruber SA, Batchu RB. (2011) "MicroRNA-101 Inhibits Growth of Epithelial Ovarian Cancer by Relieving Chromatin-Mediated Transcriptional Repression of p21waf1/cip1." Pharm Res. 28: 3079-90.
5.) Guo AM, Liu X, Al-Wahab Z, Maddippati KR, Ali-Fehmi R, Scicli AG, Munkarah AR. (2011) "Role of 12-lipoxygenase in regulation of ovarian cancer cell proliferation and survival." Cancer ChemotherPharmacol.68: 1273-83.
6.) Selvendiran K, Ahmed S, Dayton A, Kuppusamy ML, Tazi M, Bratasz A, Tong L, Rivera BK, Kalai T, Hideg K, Kuppusamy P. (2010) "Safe and targeted anticancer efficacy of a novel class of antioxidant-conjugated difluorodiarylidenylpiperidones: differential cytotoxicity in healthy and cancer cells." Free RadicBiol Med.48: 1228-35.
和数量充足,干细胞/原代细胞上清可在提取前采用超滤管(100KD)浓缩 3~5 倍后再提取。 3、使用EPF柱时堵塞如何处理? 答:过滤柱的孔径在 220nm 左右。如果出现堵塞情况,可以将柱子旋转 180 度再次离心。若上室还有残留液体,需要使用新的 EPF 柱,该柱子可以单独购买(货号 UR90102)。 4、沉淀法的具体原理是什么? 答:将高亲水性聚合物(聚乙二醇等)添加到含外泌体的溶液中后,外泌体周围的水分子被聚合物束缚,降低了外泌体的溶解度并诱导其随后的沉淀,使外泌体在低速离心下可以很容易
能准确地模拟体内环境。而且3D细胞培养又比2D细胞培养更能体现体内的环境。因此,用原代细胞构建的3D培养是模拟体内复杂生物过程的最准确方法。 3D体外模型的建立对细胞培养基提出了更严格的要求。首先,该培养基必须促进细胞终端分化,细胞分层并形成多层的结构。然而,它又必须能维持基底层的增殖细胞,这样才能持续供应模型所需的未分化细胞。这种矛盾的需求是传统的培养基无法满足的。新的3D培养基配方独特,能同时满足3D模型的增殖和分化。 CELLnTEC新的3D表皮角质形成细胞培养基
基的选择总结如下心得,以供大家参考: 1) 肿瘤细胞系及悬浮细胞的培养基选择 肿瘤细胞系是最容易饲养的细胞,对胎牛血清要求不高,甚至用新生牛血清就可以了,没有必要用高质量的Life或澳洲胎牛血清。比如,四季青、吉泰、WHB等国产牌子的血清完全可以胜任。 对于CHO悬浮细胞,甚至不用添加血清,选择将DMEM作为基本培养基,并添加培养细菌用的有酵母抽提物或植物抽提物就完全可以胜任,超便宜,可以配500 ML不会超过100元。 2) 原代哺乳细胞培养基的选择 对于需要扩增的原代哺乳
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