- ￥1500 - 2500
- 百欧泰
- BPVA-5000-Protein A
- 2025年08月01日
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- 技术资料
- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
5ml/10ml
| 规格： | 5ml | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 10ml | 产品价格： | ￥2500.0 |
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文献和实验Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
diameter (Spherotech, SVP‐50‐5) Bead coupling buffer (BCB; see recipe ) Biotinylated RGS protein (see protocol
In-gel Tryptic Digest for Protein ID by Mass Spectrometry
and shrink gel pieces.3.Remove acetonitrile with pipet.Speed-vac to dryness for 10 minutes.4.Swell gel particles in 150 μl 10 mM DTT in 100 mM NH4HCO3.Incubate 1 hr at 56℃.5.Cool to RT.Replace DTT solution with 150 μl 55 mM iodoacetamide in 100 mM NH4HCO3
Experimental Applications: Human Acetylcholinesterase as a Model Nervous System Protein
AChE. At 10−4 M BW, endogenous oocyte AChE activity (Gundersen and Miledi, 1983) was only 50% inhibited, whereas rHAChE was essentially 100% inhibited at this concentration. A similar differential sensitivity of the amphibian and mammalian enzymes
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