- ￥1868
- abinScience
- France
- MF989012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Mouse RNASEH2 Protein, N-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Mouse RNASEH2 Protein, N-His |
|---|---|
| Catalog No. | MF989012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q9CWY8 |
| Reconstitution | Reconstitute in sterile water for a stock solution. |
| Alternative Names | Ribonuclease H2 subunit A, RNase H2 subunit A, EC:3.1.26.4, Ribonuclease HI large subunit, RNase HI large subunit, Ribonuclease HI subunit A, Rnaseh2a |
| Species | Mus musculus (Mouse) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
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Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• Clinical and molecular phenotype of Aicardi-Goutieres syndrome., PMID:17846997
• RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases., PMID:19034401
• Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition., PMID:29315404
• Depletion of RNASEH2 Activity Leads to Accumulation of DNA Double-strand Breaks and Reduced Cellular Survivability in T Cell Leukemia., PMID:35500843
• Defining the RNaseH2 enzyme-initiated ribonucleotide excision repair pathway in Archaea., PMID:28373277
• High Flexibility of RNaseH2 Catalytic Activity with Respect to Non-Canonical DNA Structures., PMID:34068992
• AGS, SLE, and RNASEH2 mutations: translating insights into therapeutic advances., PMID:25500879
• Second generation lethality in RNAseH2a knockout zebrafish., PMID:39217460
• Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition., PMID:30532030
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利：N-端，C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合，你可以将目的基因替换MCS―Intein2―几丁质结合蛋白，如果想做C端融合表达，就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS，不用分2个载体；而且做过克隆的人会很有体会：很多时候双酶切载体，由于2个酶切位点都多克隆位点上，没有小片断切下来，所以是否成功双酶切就不好判断，如果用Twin载体就会切下一个片断
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