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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant IBV S1-NTD Protein, N-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug

| Product name | Recombinant IBV S1-NTD Protein, N-His |
|---|---|
| Catalog No. | VK710022 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P12651 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S |
| Species | Avian infectious bronchitis virus |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• Regulation of chromatin structure and function: insights into the histone chaperone FACT., PMID:33590780
• SARS-CoV-2 Spike N-Terminal Domain Engages 9-O-Acetylated α2-8-Linked Sialic Acids., PMID:37104622
• Deep mutagenesis scanning using whole trimeric SARS-CoV-2 spike highlights the importance of NTD-RBD interactions in determining spike phenotype., PMID:37535672
• Increased sphingoid base-1-phosphates and failure of neural tube closure after exposure to fumonisin or FTY720., PMID:22991331
• Glycan masking of NTD loops with a chimeric RBD of the spike protein as a vaccine design strategy against emerging SARS-CoV-2 Omicron variants., PMID:39192804
• Caenorhabditis elegans BRICHOS Domain-Containing Protein C09F5.1 Maintains Thermotolerance and Decreases Cytotoxicity of Aβ42 by Activating the UPR., PMID:29534049
• Secondary structure and transmembrane topology analysis of the N-terminal domain of the inner membrane protein EccE1 from M. smegmatis using site-directed spin labeling EPR., PMID:33245893
• The SARS-CoV-2 spike N-terminal domain engages 9- O -acetylated α2-8-linked sialic acids., PMID:36263070
• New Tools and Nuanced Interventions to Accelerate Achievement of the 2030 Roadmap for Neglected Tropical Diseases., PMID:38662694
信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
如下:几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利:N-端,C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合,你可以将目的基因替换MCS―Intein2―几丁质结合蛋白,如果想做C端融合表达,就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS,不用分2个载体;而且做过克隆的人会很有体会:很多时候双酶切载体,由于2个酶切位点都多克隆位点上,没有小片断切下来,所以是否成功双酶切就不好判断,如果用Twin载体就会切下一个片断
颜宁团队 2021 首秀+ 2020 年度文章赏析,看大牛如何玩转结构生物学!
1 类似结构,揭示了连接 N 端结构域(NTD)和跨膜固醇传感结构域(SSD)的隧道结构;在 pH 5.5 时,NTD 表现出两个构象,表明胆固醇向隧道输送的运动。在通道的膜边界发现了一个假定的胆固醇分子,TM2 向 SSD 上的表面袋形成。最后,在 pH 5.5 时获得了分辨率为 4.0 的 NPC1-NPC2 复合物的结构,阐明了胆固醇从 NPC2 转移到 NPC1(NTD)的分子基础。 图片来自:Cell 6. 设计新型膜蛋白研究流程,助力膜蛋白结构研究2020 年 7 月 17 日,颜宁团队
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