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- 技术资料
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
多克隆
- 抗体英文名:
GFP-Tag Mouse Polyclonal Antibody
- 规格:
支
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Product Name: GFP Mouse Polyclonal Antibody
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000 IHC: 1:200 IF: 1:200 IP:1:100
Optimal dilutions should be determined by the end user.
Specificity: The GFP tag antibody can recognize GFP,EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
Alternative Names:Green fluorescent protein
Form: Liquid
Reactivity:ALL

Western blot analysis of GFP Recombinant protein with BE2003 diluted at
1) 1:5,000
2) 1:10,000
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
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