CLP1231 HCV Core Protein (59-6

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

2 O h 4.11 ml 4.02 ml 3.93 ml 3.84 ml 3.76 ml 3.59 ml Light gel buffer 2.33 ml 2.33 ml 2.33 ml 2.33 ml 2.33 ml 2.33 ml Gel A/B 474 µl 553 µl 632 µl 711 µl 789 µl 947 µl Gel A 51 µl 60 µl 68 µl 77 µl 86 µl 103 µ

Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions

) at the resulting protein?protein interface is measured. This version of the experiment yields additional kinetic information that can help to define the solvent?inaccessible ?core? of the interface. Keywords: antibody; epitope; hydration

庚型肝炎病毒致病性研究进展

，二者均为有包膜的正链RNA病毒。随后Simons用重组蛋白建立的ELISA技术检测非A-E肝炎病人的血清，发现一名西非病人血清抗GBV-A和GBV-B抗体反应阳性，用代表性差异分析扩增得到的为一个黄病毒样基因产物。该产物在核苷酸水平上与GBV-A，GBV-B和HCV的同源性分别为59％，47.9％和53.7％，而在氨基酸水平上同源性为64.2％，50.4％和57.3％。经检索发现，该基因序列与已发表且被公认的基因序列无任何同源性，因此将该序列所代表的病毒命名为GBV-C〔2〕。1995年美国Kim