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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
20次
产品简介:
品牌:ZYMO RESEARCH/简石
货号:R1038
产品名称:ZR Urine RNA Isolation™ Kit (20 Preps) w/ Zymo-Spin™ IC Columns (Capped)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪653年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Total RNA Isolation From Laser dissected samples
($13,000 x g) to completely dry the HiBind® matrix. 10. Elution of RNA. Transfer the column to a clean 1.5 mL centrifuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water (supplied with kit). Make sure to add water
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Protocol for 5' End Labeling RNA
(available in the KinaseMax™ Kit) or by the following protocol: 3) To the above reaction, add: 125 µl water 15 µl 5.0 M ammonium acetate or 3.0 M sodium acetate 150 µl phenol/chloroform Phenol extract by spinning the tube at room
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