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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4 C / RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
10次
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D5209
产品名称:Zymo-Spin™ ChIP Kit (10 preps) Mechanical Shearing Protocol '*'
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪457年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
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