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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
25ml
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D7010-2-25
产品名称:DNA/RNA Prep Buffer (25 ml)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪553年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
onto a formaldehyde gel. Electrophorese overnight at 20 volts. Blot onto nitrocellulose. Probe with DNA fragment of choice. Expose to film. Yields are typically on the order of 1-2 mg total RNA from an overnight 50 ml culture arising from an average slant
Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Centrifuge at maximum speed ($13,000 x g) for 3 minutes at room temperature Carefully discard supernatant. 24. Air dry the DNA pellet by inverting the tube on a absorbent paper for 5-10 minutes. 25. Add 100ul EB Buffer to the tube and incubate
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