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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
2ml
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D5014-2
产品名称:Human HCT116 DKO Methylated DNA (5 μg / 20 μl)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪402年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Clonality - X Chromosome Inactivation Assay
) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies
Methylated DNA Immunoprecipitation (MeDIP)
that contain methylated cytosines in their genome (human, mouse, Arabidopsis thaliana , Neurospora crassa ). Resuspend the cell pellet or the homogenized tissue in 300 µl TE in a 2 ml eppendorf tube (the volume should be increased for big cell
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
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![5-Methylcytosine dNTP Mix [10 mM] ^](https://img1.dxycdn.com/p/s14/2025/0728/935/8849535966970379491.png!wh200)

