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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
100Preps
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D4015
产品名称:ZR Plasmid Miniprep™-Classic Kit (100 Preps)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪268年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
most of it will pellet along with the bacterial cell wall. However the relative purity of the plasmid DNA will drop if the plasmid is large or at a relatively low copy number in the prep. The combination of a kit prep with cesium banding eliminates this problem
Fastfilter Plasmid Maxi Kit Spin Protocol
实验步骤 Growth of bacterial culture: 1. Culture volume: Inoculate 200 ml LB/ampicillin (50 ug/ml) medium placed in a 1-4 liter culture flask with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16
Introduction Extraction and purification of plasmid DNA from E.coli can be performed on a small scale,where DNA is extracted from just 1-2 ml of bacterial culture (mini-prep), or from much larger volumes (100 ml %26ETH; maxi-prep; 1 L %26ETH
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