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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
50Pack
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D4084///D4085
产品名称:Zymo Collection Swab, 80mm break point
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪168年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
bath - set to 65°C 实验步骤 1. Place the buccal swab or paper contains dried blood spot in a 2 ml centrifuge tube and add 500 ul RS1 Buffer in the tube. If RNA-free DNA is required for downstream application, add 4 ul RNase A (25
. If precipitation can be seen at this point, break the precipitation by passing through a needle using a syringe. TIP: In most cases, 2.0 ml supernatant can easily be removed. This will require 2.0 ml of 70% ethanol. Note that depending on the sample
Points from here (point 1) up to and including point 8 are related to Preparation of rat brain cytosolTiming : 4�5 h Prepare sucrose buffer with protease inhibitors (1 μg ml−1 pepstatin A (1:1,000 vol/vol of pepstatin A stock solution) and 0.2-mM
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