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- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
250Pack
产品简介:
品牌:ZYMO RESEARCH/简石
货号:C1005-250
产品名称:Zymo-Spin™ III Columns (250 Pack) (Uncapped)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪122年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
41.iii) LiCl buffer for ChIP Lymphocyte separation medium (LSM) Lymphocytes, human leukapheresis pack from a blood bank The pack contains leukocytes enriched by apheresis from human peripheral blood. Micrococcal nuclease
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
break up the colonyand resuspend the cells.ii. Incubate at 30°C overnight with shaking at 230–250 rpm.iii. Spin down the cells by centrifuging at 14,000 rpm for 5 min.iv. Carefully pour off the supernatant and resuspend pellets in the residual liquid
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