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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
25ml
产品简介:
品牌:ZYMO RESEARCH/简石
货号:A1001-25
产品名称:Ampicillin (25 ml) 100 mg/ml (5 x 5 ml) ^
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪97年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
实验试剂 1. Isopropanol (100%) 2. 70% ethanol 3. Using different volume of Solution according blood volume as following: 实验设备 1. Centrifuge capable of 2,000 x g 2. Nuclease-free
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
. Then, the supernatant of each tube is transfered into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed
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