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- 库存:
99
- 供应商:
卡梅德生物科技(天津)有限公司
- 规格:
10UG/50UG
| 规格: | 10UG | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 50UG | 产品价格: | ¥4000.0 |
产品编号:COV424;
宿主:E.coli;
应用:;
纯度:≥95% as determined by SDS-PAGE;
内毒素水平:<0.1 EU/ug by LAL;
生物活性:
卡梅德生物科技(天津)有限公司作为国家高新技术企业,专注于为全球科学家和研究机构提供天然蛋白提取与发酵、重组蛋白定制表达服务,单、多克隆抗体开发,噬菌体文库构建与筛选服务;同时,基于我公司成熟的五大核心技术服务平台,成功研发出了众多用于科学研究的重组蛋白、抗体、抗体药物靶点蛋白、工业酶、诊断原料等相关试剂。卡梅德生物的五大核心平台主要包括噬菌体抗体展示技术平台,抗体工程技术平台,蛋白表达与纯化服务技术平台,蛋白批量发酵平台和稳定细胞系筛选平台与基础分子生物平台。
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
of interest, but some affinity tails are able to be linked to either the N- or C-terminus of the protein of interest. The choice of affinity tail to use for the expression of any particular protein is empirical since the factors leading to the high expression of recombinant
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