- ￥1092 - 21120
- Solarbio
- P01278
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥21120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥14784.0 |
| 规格： | 50ug | 产品价格： | ￥3276.0 |
| 规格： | 10ug | 产品价格： | ￥1092.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Tryptophan synthase alpha chain;trpA; |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of PBS, pH7.4. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan.It is commonly found in Eubacteria，Archaebacteria，Protista，Fungi，and Plantae，but is absent from animals such as humans.Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction.Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Isoleucine Ile I Leucine Leu L Lysine Lys K Methionine Met M Phenylalanine Phe F Proline Pro P Serine Ser S Threonine Thr T Tryptophan Trp W Tyrosine Tyr Y Valine Val V Biomek configuration for single stranded
Tris-HCl, pH 8.0 平衡上样，NaCl 浓度梯度洗脱，获得了“天然”状态的活性蛋白质。 在IEC 中，变性剂的浓度梯度并不能每次都获得高的活性回收，例如α乳白蛋白的收率只有10% 左右。原因可能是折叠的中间体很难从层析介质上洗脱出来。为了避免折叠中间体在层析介质上的堆积，我们发展了两种缓冲液系统的复性方法[26] 。溶解的蛋白质吸附在高浓度变性剂缓冲液平衡的层析柱上，梯度降低变性剂的浓度，同时梯度增加盐离子的浓度，这样可以使蛋白质自发折叠的同时，离开原先吸附的位点，向柱下端移动
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
_004889 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2 (ATP5J2), mRNA 1513 NM_003769 Homo sapiens splicing factor, arginine/serine-rich 9 (SFRS9), mRNA 1207 NM_003910 Homo sapiens maternal G10 transcript
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