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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥8640.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥6048.0 |
| 规格: | 50ug | 产品价格: | ¥780.0 |
| 规格: | 10ug | 产品价格: | ¥312.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Ubiquitin-Conjugating Enzyme E2 D4;HBUCE1;Ubiquitin Carrier Protein D4;Ubiquitin-Protein Ligase D4;UBE2D4;UBCH5D |
| 中文名称 | Recombinant Human UBE2D4/Ubiquitin-conjugating enzyme E2 D4/UbcH5d |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family.UBE2D4 has been proposed to participate in Ubl conjugation pathway.UBE2D4 takes part in post-translational protein modification,protein K6-linked ubiquitination,protein K11-linked ubiquitination,protein K27-linked ubiquitination,protein K29-linked ubiquitination,protein K48-linked ubiquitination,and protein K63-linked ubiquitination.UBE2D4 regulate of protein metabolic process.UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.In vitro,UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues,but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Studies of the Ubiquitin Proteasome System
( protocol 34 ) E2‐UbcH5B ( protocol 37 ) GST‐ubiquitin ( protocol 29 ) Human APP‐BP1/Uba3 (NEDD8 E1; protocol 21 ) Ubc12 (E2
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外,有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4) 利用Rho1D4纯化膜蛋白图解简述步骤1:结合:将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2:清洗:将杂蛋白和其他裂解液成分洗去,留下与亲和基质结合的目标蛋白。 步骤3:洗脱:过量Rho1D4肽与基质竞争性结合,释放出目标蛋白在洗脱液中收集。 表
Proteasomes that Lack Endogenous USP14 and are Devoid of UB‐AMC Hydrolysis Activity Support Protocol 2: Purification of Recombinant Human USP14 from E. coli Support Protocol 3: Synthesis and Purification of Ubiquitin
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