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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-5~-20℃,避光储存
- 保质期:
12个月
- 英文名:
Retinoic Acid (ATRA) Solution (100 mM)
- 库存:
99
- 供应商:
武汉普诺赛生命科技有限公司
- CAS号:
无
- 规格:
1mL
产品描述:
在干细胞研究中,Y-27632(10 µM)处理人胚胎干细胞1小时,能够有效抑制干细胞的凋亡,显著提高克隆形成效率,并延长细胞的传代能力。此外,Y-27632通过调控上皮-间充质转化(EMT)样过程,能够选择性地诱导人诱导多能干细胞(hiPSCs)向间胚层谱系分化,为干细胞定向分化研究提供了重要工具。
优势特点:
Y-27632是一种ATP竞争性的ROCK(Rho-associated protein kinase)选择性抑制剂,具有高效、细胞可渗透性、可逆性和高度选择性。其对p160ROCK(Ki=140 nM)和ROCK-II(IC₅₀=800 nM)表现出显著的ATP竞争性抑制作用,同时对PRK2(ICIC₅₀=600 nM)也有一定的抑制效果。Y-27632通常在细胞种板培养的初始阶段添加,后续换液培养时可不使用。
产品储存方法:
将维生素A酸溶液(100 mM) -5~-20℃,避光储存,有效期为12个月
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文献和实验Introduction to QM/MM Simulations
Hybrid quantum mechanics/molecular mechanics (QM/MM) simulations have become a popular tool for investigating chemical reactions in condensed phases. In QM/MM methods, the region of the system in which the chemical process takes place
QM and QM/MM Simulations of Proteins
optimization and dynamic simulations of peptides and proteins. In this chapter, we introduce methodological developments such as the QM/MM framework and linear-scaling QM that make efficient calculations on large biomolecules possible. We identify
MM-GB/SA Rescoring of Docking Poses
binding modes, but struggle when predicting the corresponding binding affinities. The rescoring of docking poses using the MM-GB/SA technique has emerged as an important computational approach in structure-based lead optimization as it provides
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