IL0510 番茄红素 免疫学/炎症 索莱宝

一种类胡萝卜素。所具有的长链多不饱和烯烃分子结构，使其具有很强的消除自由基能力和抗氧化能力。目前对其生物学作用的研究主要集中在抗氧化、降低心血管疾病风险、减少遗传损伤和抑制肿瘤发生发展等方面。

使用本产品的案例

In Vitro

Cell（mouse microvascular cerebral endothelial cells/bEnd.3；lycopene溶解在THF（含0.25%BHT）中，母液浓度为4 mM ）

For the in vitro experiment, we grew mouse microvascular cerebral endothelial cells (bEnd.3) in Dulbecco’s modified Eagle's medium (DMEM) supplemented with 10% FBS and 1% 100 U/mL penicillin and 100 U/mL streptomycin.  Cultures were maintained under 5% CO2 at 37℃.    We dissolved lycopene (Solarbio, China) in tetrahydrofuran (THF) containing 0.25% butylated hydroxytoluene (BHT) and stored it at -80℃ in 4 mM stock.    On Day 5 of bEnd. 3 culture, the medium was replaced with pre-warmed PBS and then divided into a control group (solvent group), Ly294002 treatment group (10 mM;    inhibitor group), and lycopene t Ly294002 group (lycopene t inhibitor group). The control group was treated with the same dose of FBS and double antibody (0.25% BHT in THF) to offset any possible interference. The lycopene t inhibitor group was pretreated with Ly294002 for 30 min and then treated with lycopene for 24 h.

来源文献：Xu XD, Teng Y, Zou JY, Ye Y, Song H, Wang ZY. Effects of lycopene on vascular remodeling through the LXR-PI3K-AKT signaling pathway in APP/PS1 mice. Biochem Biophys Res Commun. 2020 Jun 4;526(3):699-705. doi: 10.1016/j.bbrc.2020.02.063. Epub 2020 Apr 3. PMID: 32253029.

Cell（H9c2 cells，2.5~40 μM LP, 12h；选择10 μM LP作为实验组）

Firstly, H9c2 cells were randomly divided into 8 groups at the first phase as follows: (i) control group (Control): H9c2 cells were incubated in normoxic conditions at 37°C in a cell culture incubator for 13 hrs; (ii) hypoxia/re-oxygenation group (H/R): H9c2 cells were subjected to hypoxia for 12 hrs followed by reoxygenation for 1 hr; (iii) solvent group (Vehicle): H9c2 cells were pretreated with 0.1% DMSO for 12 hrs and underwent HR group procedures; (iv)–(viii) 2.5 μM LP pretreatment group (LP 2.5 μM), 5 μM LP pretreatment group (LP 5 μM), 10 μM LP pretreatment group (LP 10 μM), 20 μM LP pretreatment group (LP 20 μM), 40 μM LP pretreatment group(LP 40 μM): H9c2 cells were pretreated with LP at the dose of 2.5 μM, 5 μM, 10 μM, 20 μM and 40 μM for 12 hrs and underwent HR group procedures.

In the second phase (according to the result of first phase), 10 μM LP was selected to explore the mechanism further.  Therefore, H9c2 cells were further divided into 4 groups as follows: (i) Control group;  (ii) HR group;  (iii)LP 10 μM group;  (iv) LP 10 μM combined with ATR pretreatment group (LP 10 μM + ATR): H9c2 cells were pretreated with 10 μM LP and 20 μM ATR for 12 hrs and underwent HR group procedures.

来源文献：Li X, Jia P, Huang Z, Liu S, Miao J, Guo Y, Wu N, Jia D. Lycopene protects against myocardial ischemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening. Drug Des Devel Ther. 2019 Jul 11;13:2331-2342. doi: 10.2147/DDDT.S194753. PMID: 31371925; PMCID: PMC6635826.

In Vivo

Mice（9-month-old APP/PS1 mice，4 mg/kg lycopene）

We randomly assigned 12 APP/PS1 transgenic mice to two groups: APP/PS1+solvent (APP/PS1; AD group; n=6) and AD+4 mg/kg lycopene (LY; drug group; n = 6). Similarly, we randomly selected six WT C57/BL6J mice as the wild control group (WT group).

来源文献：Xu XD, Teng Y, Zou JY, Ye Y, Song H, Wang ZY. Effects of lycopene on vascular remodeling through the LXR-PI3K-AKT signaling pathway in APP/PS1 mice. Biochem Biophys Res Commun. 2020 Jun 4;526(3):699-705. doi: 10.1016/j.bbrc.2020.02.063. Epub 2020 Apr 3. PMID: 32253029.

Rat（10~40 mg/kg LP，1次/天，共5 d，腹腔注射；选择40 mg/kg LP作为实验组；溶剂：玉米油）

In the first phase, 30 rats were divided into the following 5 groups with 6 rats per group: (i) ischemia-reperfusion group (IR): As described above;   (ii) solvent group (Vehicle): The rats were pretreated with 2 mL medicinal corn oil by intraperitoneal injection once per day for 5 days, and underwent IR procedures;   (iii–v) 10 mg/kg LP pretreatment group (LP 10 mg/kg), 20 mg/kg LP pretreatment group (LP 20 mg/kg), 40 mg/kg LP pretreatment group (LP 40 mg/kg): The rats were pretreated with 10, 20, 40 mg/kg LP by intraperitoneal injection once per day, for a total of 5 days, and underwent IR group procedures.

In the second phase (according to the results in the first phase), 40 mg/kg LP was selected as the most appropriate dose.   A total of 80 rats were divided into the following 4 groups with 20 rats per group in the second phase: (i) IR group;   (ii) Vehicle group;   (iii) LP 40 mg/kg group;   (iv) 40 mg/kg LP in combination with ATR pretreatment group (LP 40 mg/kg + ATR): 5 mg/kg ATR was injected intraperitoneally 30 mins prior to extraction of the heart, and the remainder of the procedure was as described for the LP 40 mg/kg group.

来源文献：Li X, Jia P, Huang Z, Liu S, Miao J, Guo Y, Wu N, Jia D. Lycopene protects against myocardial ischemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening. Drug Des Devel Ther. 2019 Jul 11;13:2331-2342. doi: 10.2147/DDDT.S194753. PMID: 31371925; PMCID: PMC6635826.

Mice（healthy male kunming mice (6 weeks old) weighing 23–28 g；5 mg/kg LYC（1 mg/mL的悬浮液），灌胃；溶剂：橄榄油）

After an acclimatization period of a week, the mice were divided into four groups, ten in each. The mice were treated for 30 days as follows: Group 1 (CG) was orally administered with the vehicle (0.2 mL olive oil). Group 2 (AG) was orally administrated with AFB1 (≥ 99.8%) at 0.75 mg/kg.  Group 3 (ALG) were treated with LYC (≥ 98%, Solarbio, Beijing, China) at 5 mg/kg 2 h prior to AFB1 administration, then given AFB1 at 0.75 mg/kg. Group 4(LG) was orally administrated with LYC at 5 mg/kg. AFB1 and LYC were administered by gavage in olive oil and made into suspension at the concentrations of 0.2 mg/mL and 1 mg/mL, respectively.  The general health was monitored daily.

来源文献：Zhang J, Wang P, Xu F, Huang W, Ji Q, Han Y, Shao B, Li Y. Protective effects of lycopene against AFB1-induced erythrocyte dysfunction and oxidative stress in mice. Res Vet Sci. 2020 Apr;129:103-108. doi: 10.1016/j.rvsc.2020.01.015. Epub 2020 Jan 13. PMID: 31954314.

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Metabolic Engineering of Yarrowia lipolytica for Zeaxanthin Production

成员:Guilin Zhang, Jing Chen, Yongzhen Wang, Zhen Liu, Xiangzhao Mao

论文因子:6.1 发表期刊:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY pmid:37676277

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Lycopene aggravates acute gastric injury induced by ethanol

成员:Chen X; Zhao Y; Liu K; Li Z; Tan X; Wang Y; Gao N; Liu C; Fang X; Wang Y.

论文因子:5 发表期刊:Frontiers in Nutrition pmid:34485361

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Protective effects of lycopene against AFB1-induced erythrocyte dysfunction and oxidative stress in mice

成员:Zhang J; Wang P; Xu F; Huang W; Ji Q; Han Y; Shao

论文因子:2.372 发表期刊:Res Vet Sci pmid:31954314

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Effects of lycopene on vascular remodeling through the LXR-PI3K-AKT signaling pathway in APP/PS1 mice

成员:Xu XD; Teng Y; Zou JY; Ye Y; Song H; Wang ZY

论文因子:3.307 发表期刊:Biochem Biophys Res Commun pmid:32253029

【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】

标题:Lycopene protects against myocardial ischemia-reperfusion injury by inhibiting mitochondrial permeability transition pore opening

成员:Li X; Jia P; Huang Z; Liu S; Miao J; Guo Y; Wu N;

论文因子:3.349 发表期刊:Drug Des Devel Ther pmid:31371925

基本信息
CAS	No.502-65-8
中文名称	番茄红素
英文名称	Lycopene
别名	ψ,ψ-Carotene;番茄红素
分子式	C40H56
分子量	536.87
溶解性	Soluble in Chloroform ≥1mg/mL(该产品极易被氧化，建议现配现用)
纯度	HPLC≥90%
外观(性状)	Red to reddish brown Solid
储存条件	Powder: -20℃，1 year
EC	EINECS 207-949-1
MDL	MFCD00017350
SMILES	C/C(C)=C\CC/C(C)=C/C=C/C(C)=C/C=C/C(C)=C/C=C/C=C(/C=C/C=C(C)/C=C/C=C(CC/C=C(C)\C)\C)C
靶点	Reactive oxygen species (ROS)
通路	Immunology & Inflammation
背景说明	一种类胡萝卜素。所具有的长链多不饱和烯烃分子结构,使其具有很强的消除自由基能力和抗氧化能力。
生物活性	Lycopene是番茄，番茄产品和其他红色水果和蔬菜中天然存在的类胡萝卜素; 具有抗氧化作用。[1-5]
In Vitro	由于人类无法从头合成番茄红素，因此必须从饮食中充分摄取番茄红素以从其健康促进效果中受益。番茄红素在1.25μM的生理相关浓度下显着抑制前列腺癌和乳腺癌细胞生长，并且还导致细胞中κB磷酸化抑制剂减少30-40％[1]。西红柿中一种主要类胡萝卜素的番茄红素的摄入量增加，作为全反式异构体消耗减少酒精诱导的2E1细胞凋亡并降低前列腺癌，肺癌和消化道癌症的风险。番茄红素通过作为单线态分子氧和过氧自由基清除剂起到防光氧化过程的作用，并且可以与其他抗氧化剂协同作用[2]。作为类胡萝卜素的番茄红素可以以三种不同的机制与活性氧物质(ROS)的类型反应：I)通过电子转移，II)通过氢原子转移或III)通过加合物形成。番茄红素能够主要通过物理猝灭使单线态氧失活[3]。番茄红素通过抑制PKC途径引起的NADPH氧化酶降低SK-Hep-1细胞中的ROS产生[5]。
细胞实验	番茄红素是人血浆中最主要的类胡萝卜素，半衰期约为2-3天[2]。番茄红素或加工番茄可能导致血管壁内膜中层厚度减少[3]。番茄红素对大鼠肾上腺皮质中ATZ诱导的毒性具有保护作用。这些影响可能归因于番茄红素的抗氧化特性及其激活Nrf2 / HO-1途径的能力[4]。番茄红素可改善肝毒性，起抗氧化剂作用，降低GSSG，调节tGSH和CAT水平，减少氧化损伤[5]。
细胞实验	用(0，0.5，1.25，2.5和5μM)番茄红素处理PC3细胞和MDA-MB-231细胞48小时。使用比色MTS测定方法测量细胞存活/生长。向每个孔中加入MTS-PMS复合物(20μL)。活细胞的催化活性导致甲dye染料产生，然后量化。将细胞与染料一起孵育1小时，然后在分光光度计上读取492nm处的吸光度[1]。
动物实验	大鼠：番茄红素溶解在玉米油中。将35只成年雄性白化病大鼠随机分成5组：未处理对照组，媒介物对照组(每天接受0.5毫升玉米油)，番茄红素(10毫克/千克体重/天)，ATZ(溶于0.5毫升玉米油300毫克/千克)bw /天)和ATZ +番茄红素。所有治疗均通过口服强饲法治疗4周[4]。
数据来源文献	[1]. Assar EA， et al. Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells. Tumour Biol. 2016 Jul; 37 (7) :9375-85. [2]. Tapiero H， et al. The role of carotenoids in the prevention of human pathologies. Biomed Pharmacother. 2004 Mar; 58 (2) :100-10. [3]. Müller L， et al. Lycopene and Its Antioxidant Role in the Prevention of Cardiovascular Diseases-A Critical Review. Crit Rev Food Sci Nutr. 2016 Aug 17; 56 (11) :1868-79. [4]. Abass MA， et al. Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway. Environ Sci Pollut Res Int. 2016 Aug; 23 (15) :15262-74. [5]. Bandeira AC， et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice. Chem Biol Interact. 2017 Feb 1; 263:7-17.
单位	瓶