- ￥3150
- ACROBiosystems
- GMP-CM3101
- 2025年07月23日
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- 详细信息
- 技术资料
- 规格：
1L
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产品描述（Product Details）
CelThera™ GMP T Cell Expansion Medium is a serum-free culture medium specifically developed to support human T cell culture. It is a serum-free, animal origin-free T cell maintenance and expansion medium produced under GMP conditions.
Compared to traditional culture media or xeno-free culture media, animal origin-free culture media can better reduce the risk of introducing potential pathogenic microorganisms during culture process, improve batch-to-batch consistency, and prevent T cell overactivation by undefined components in the serum.
CelThera™ GMP T Cell Expansion Medium does not require the addition of any serum or serum replacements and also maintains the high fold expansion of T cells. If users choose to add serum or serum replacement, the dosage should be determined by specific T cell applications.
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优势特色（Features）
- Serum-free, animal origin-free (AOF), and exogenous growth factors free.
- Designed to support low-density seeding and high fold expansion of T cells.
- Suitable for large-scale T cell expansion.
- No additional serum or serum replacements needed.
- T cell phenotypes similar to media supplemented with serum or serum replacement.
- Contains only recombinant proteins as components, and no antibiotics included in formulation.
- Produced according to current GMP guidelines.
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存储（Storage）
The CelThera™ GMP T Cell Expansion Medium is stable for 18 months when stored under 2-8°C, protect from light.
The CelThera™ GMP T Cell Expansion Supplement is stable for 12 months when stored under -20°C or below, protect from light.
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应用数据（Application Data）
T cell expansion rate and cell viability in various media.
T cells from PBMCs of 3 different donors were activated and cultured for 11 days in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). Cell count and viability were performed on day 6, day 8 and day 11 by trypan blue staining. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3101) had a faster proliferation rate and higher viability than that of the other two media. -
Tcm ratio in various media.
T cells from PBMCs of 3 different donors were activated and cultured in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). Tcm percentage (CD45RO+/CCR7+) was determined by flow cytometry when cells reached about 50-fold expansion. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3101) had a higher percentage of central memory T cells than that of the other two media. -
Lentivirus transduction efficiency in various media.
T cells from PBMCs were activated and cultured in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). 24 hours after activation, the cells were transduced with pLenti-CMV-EGFP-puro lentivirus (MOI=3 or 6). 24hrs after transduction, the lentivirus was removed by centrifugation. Then, the cells were cultured for 48hrs and the CD3+eGFP+ population was detected by flow cytometry. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3101) had a similar lentiviral transduction efficiency to that of the other medium.
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多供体验证数据（Multiple Donor Verification）
Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable to Competitor L +2.5% SR. Notably, the cells exhibit better expansion in CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101).
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大规模培养数据（Large-scale Culture Verification）
Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody, and CelThera™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.
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